Circ-MALAT1靶向miR-101抑制膀胱癌细胞系SW780侵袭和迁移

目的 探讨circ-MALAT1靶向miR-101抑制膀胱癌细胞侵袭和迁移.方法 将膀胱癌细胞系SW780分为si-NC组(转染si-NC)、si-circ-MALAT1组(转染si-circ-MALAT1)、miR-NC组(转染miR-NC)、miR-101组(转染miR-101 mimics)、(si-circ-M...

Circ-MALAT1 targeting miR-101 inhibits invasion and migration of bladder cancer cell line SW780

Objective To explore the function of circ-MALAT1 targeting miR-101 in the inhibition of the invasion and migration of bladder cancer cells.Methods Bladder cancer cell line SW780 was divided into si-NC group(transfection si-NC),si-circ-MALAT1 group(transfection si-circ-MALAT1),miR-NC group(transfection miR-NC),miR-101 group(transfection miR-101 mimics),si-circ-MALAT1+anti-miR-NC group(co-transfection si-circ-MALAT1 and anti-miR-NC)and si-circ-MALAT1+anti-miR-101 group(co-transfection si-circ-MALAT1 and anti-miR-101).RT-qPCR was used to examine the expression of circ-MALAT1 and miR-101;Transwell method was used to detect cell invasion and migration;The matrix metalloproteinase 2(MMP-2)and matrix metalloproteinase 9(MMP-9),phosphorylated phosphatidylinositol 3-kinase(p-PI3K),phosphorylated protein kinase B(p-AKT)protein expression were measured by Western blot;Dual luciferase reporter gene experiment was applied to detect the targeting relationship between circ-MALAT1 and miR-101.Results Compared with adjacent tissues,the expression of circ-MALAT1 in bladder cancer tissue was significantly increased(P<0.05)and the expression of miR-101 was significantly decreased(P<0.05).Compared with the si-NC group,the expression of circ-MALAT1 in the si-circ-MALAT1 group was significantly reduced(P<0.05)and the counting number of invasion and migrating cells was significantly reduced(P<0.05),the expression of p-PI3K and p-AKT protein was significantly reduced(P<0.05).Compared with the miR-NC group,the expression of miR-101 in the miR-101 group was significantly increased(P<0.05)and the number of invasion as well as migrating cells were significantly reduced(P<0.05).Compared with the(si-circ-MALAT1+anti-miR-NC)group,the(si-circ-MALAT1+anti-miR-101)group showed more cell invasion and migration(P<0.05),the expression of p-PI3K and p-AKT protein was significantly increased(P<0.05).Conclusions Reduction of circ-MALAT1 expression and up-regulation miR-101 inhibit the invasion and migration of bladder cancer cells line SW780.The mechanism may be related to the PI3K/AKT signaling pathway.