| UPLC法测定人血浆中卡马西平的游离浓度 |
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| 引用本文： | 董维冲,李亚前,赵梦强,刘姣,李浩然,曹培,李德强,蒋晔,张志清.UPLC法测定人血浆中卡马西平的游离浓度[J].中国临床药理学杂志,2022(3):263-266. |
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| 作者姓名： | 董维冲 李亚前 赵梦强 刘姣 李浩然 曹培 李德强 蒋晔 张志清 |
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| 作者单位： | 1. 河北医科大学第二医院药学部;2. 河北医科大学药学院 |
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| 基金项目： | 河北省科技厅科技计划基金资助项目(19277728D)； |
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| 摘 要： | 目的 建立一种测定人血浆中卡马西平游离浓度的中空纤维离心超滤(HFCF-UF)-超高效液相色谱(UPLC)法.方法 血浆样本经中空纤维离心超滤技术预处理.色谱柱:Waters UPLC BEH C18柱(50.0 mm×2.1 mm,1.7μm),流动相:乙腈-水=35:65,流速:0.2 mL·min-1,柱温:30...
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| 关 键 词： | 卡马西平 游离浓度 中空纤维 离心超滤 治疗药物监测 UPLC |
UPLC method for the analysis of free concentrations of carbamazepine in human plasma |
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| DONG Wei-chong,LI Ya-qian,ZHAO Meng-qiang,LIU Jiao,LI Hao-ran,CAO Pei,LI De-qiang,JIANG Ye,ZHANG Zhi-qing.UPLC method for the analysis of free concentrations of carbamazepine in human plasma[J].The Chinese Journal of Clinical Pharmacology,2022(3):263-266. |
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| Authors: | DONG Wei-chong LI Ya-qian ZHAO Meng-qiang LIU Jiao LI Hao-ran CAO Pei LI De-qiang JIANG Ye ZHANG Zhi-qing |
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| Institution: | (Department of Pharmacy,The Second Hospital of Hebei Medical University,Shijiazhuang 050051,Hebei Province,China;School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China) |
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| Abstract: | Objective To establish a hollow fiber centrifugal ultrafiltration(HFCF-UF)coupled ultra-high performance liquid chromatography(UPLC)method for the analysis of free carbamazepine concentration in human plasma.Methods The plasma samples were prepared by hollow fiber centrifugal ultrafiltration.Waters UPLC BEH C18 column(50.0 mm×2.1 mm,1.7μm)was used.The mobile phase consisted of acetonitrile and water(35∶65)at the flow rate of 0.2 mL·min-1.The column temperature was maintained at 30℃.The injection volume was 2μL.The linearity,precision,accuracy and stability of the method were investigated.Results The good linear relationship of free carbamazepine were obtained in the the concentration range of 0.2-50.0μg·mL-1,and the standard curve was y=2.04×104x-4.61×102(r=0.9989).The method recovery rates were 96.86%-104.89%.The absolute recovery rates were 98.40%-102.40%.The intra-day and inter-day precisions(RSD)were all less than 3.00%.Conclusion The present method is simple and accurate,suitable for the analysis of free carbamazepine concentration in human plasma. |
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| Keywords: | carbamazepine free concentration hollow fiber centrifugal ultrafiltration therapeutic drug monitoring UPLC |
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