黄芪总皂苷对脂多糖诱导的BV2小胶质细胞的抗炎机制

目的:探讨黄芪总皂苷(TAS)对脂多糖(LPS)诱导的BV2小胶质细胞炎症损伤的抗炎作用机制.方法:用CCK-8法筛选出对细胞活力无抑制的药物浓度;用浓度为1 mg/L的LPS刺激BV2细胞24 h,建立细胞炎症模型;实验分为正常组、LPS组、高剂量(75 mg/L)TAS组和低剂量(50 mg/L)TAS组;应用流式...

Anti-inflammatory mechanism of total Astragalus saponins in LPS-induced BV2 cells

AIM:To explore the effects of total Astragalus saponins(TAS)on microglia BV2 cells treated with lipopolysaccharide(LPS).METHODS:The BV2 cells were treated with LPS at a concentration of 1 mg/L for 24 h to establish an inflammatory cell model. The cells were divided into normal group,model group,high-dose(75 mg/L)TAS group and low-dose(50 mg/L)TAS group. The expression of CD16/32 was analyzed by flow cytometry. Immunofluorescence staining was used to detect the fluorescence intensity of intracellular inducible nitric oxide synthase(iNOS)and arginase-1(Arg-1). The protein levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factor-κB(NF-κB)p65 and phosphorylated NF-κB(p-NF-κB)p65 were determined by Western blot.RESULTS:Treatment with TAS at 200 mg/L exerted a significant inhibitory effect on BV2 cell viability. High-dose TAS significantly reduced the enhanced viability of BV2 cells caused by LPS.High-and low-dose TAS significantly reduced the number of CD16/32-positive cells. Immunofluorescence staining showed that TAS at low dose significantly inhibited the expression of iNOS and significantly increased the expression of Arg-1.Treatment with TAS at high or low dose suppressed the protein levels of TNF-α,IL-1β,TLR4,MyD88,NF-κB p65 and pNF-κB p65.CONCLUSION:High-and low-dose TAS may inhibit the polarization of BV2 cells to M1 phenotype,and reduce the inflammatory cytokines through the inhibition of TLR4/MyD88/NF-κB signaling pathway.