基于网络药理学探讨柴芍六君子汤防治化疗所致恶心呕吐的作用机制

目的:采用网络药理学方法,探讨柴芍六君子汤防治化疗所致恶心呕吐可能的作用机制。方法:基于中药系统药理学数据库与分析平台,检索柴芍六君子汤组成药物柴胡、白芍、党参、茯苓、白术、陈皮、半夏和甘草的化学成分和潜在靶点,选择口服生物利用度≥30%和类药性≥0.18作为化学成分筛选条件;在GeneCards数据库检索化疗所致恶心呕吐的疾病靶点;利用Cytoscape 3.6.0软件绘制“柴芍六君子汤-化学成分-靶点-化疗所致恶心呕吐”网络;使用STRING 11.0在线软件构建蛋白质-蛋白质相互作用网络并挖掘核心靶点;采用David Bioinformatics Resources数据库对该方活性成分潜在靶点网络中的蛋白进行基因本体(GO)功能富集分析和基于京都基因与基因组百科全书(KEGG)通路富集分析。结果:GO功能富集分析提示,柴芍六君子汤广泛参与了机体代谢、炎症等生物过程。KEGG生物通路富集分析显示,柴芍六君子汤参与的信号通路均与炎症相关,疾病通路主要与病毒感染、多种恶性肿瘤密切相关。结论:本研究从网络药理学角度预测了疏肝健脾法的代表方剂柴芍六君子汤疏肝行气、健脾和胃防治化疗所致恶心呕吐可能的信号通路,为后续机制研究奠定了一定的基础。

Mechanism of Chaishao Liujunzi Decoction in Prevention and Treatment of Chemotherapy-Induced Nausea and Vomiting Based on Network Pharmacology

OBJECTIVE:To probe into the potential mechanism of Chaishao Liujunzi decoction in prevention and treatment of chemotherapy-induced nausea and vomiting(CINV)based on liver-soothing and spleen-strengthening method.METHODS:The pharmacological database and analysis platform of traditional Chinese medicine system was used to search the chemical components and potential targets of drug composition of Chaishao Liujunzi decoction,including Bupleuri Radix,Paeoniae Radix Alba,Codonopsis Radix,Poria,Atractylodis Macrocephalae Rhizoma,Citri Reticulatae Pericarpium,Pinelliae Rhizoma,and Glycyrrhizae Radix et Rhizoma,oral bioavailability≥30%and drug-likeness≥0.18 were set as the screening conditions of chemical composition;the GeneCards database was used to retrieve disease targets of CINV;Cytoscape 3.6.0 software was used to draw the network of“Chaishao Liujunzi decoction-chemical components-target-CINV”;STRING 11.0 online software was used to construct a protein-protein interaction network and to mine the core targets;David Bioinformatics Resources database was used to conduct gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Gene and Genome(KEGG)pathway enrichment analysis on protein in the network of potential targets of active ingredients in the formula.RESULTS:The GO functional enrichment analysis indicated that Chaishao Liujunzi decoction had extensively participated in biological processes including body metabolism and inflammation.The KEGG pathway enrichment analysis indicated that all the signaling pathways of Chaishao Liujunzi decoction were related to inflammation,the disease pathways were mainly closely related to virus infection and various malignant tumor.CONCLUSIONS:This study predicts the possible signaling pathways of prevention and treatment of CINV of Chaishao Liujunzi decoction from the perspective of network pharmacology,a representative formula of liver-soothing and spleen-strengthening method,which laid a certain foundation for the subsequent mechanism research.