钩吻碱通过下调circ_001680抑制类风湿关节炎滑膜成纤维细胞增殖和侵袭

目的探讨钩吻碱对类风湿关节炎滑膜成纤维细胞(RASFs)增殖、迁移和侵袭的影响及可能机制。方法体外分离培养RASFs。经50、100、200μg/mL的钩吻碱干预24 h或转染circ_001680的小干扰RNA或过表达载体至RASFs后,CCK-8法和克隆形成实验检测细胞增殖;划痕实验检测细胞迁移;Transwell小室法检测细胞侵袭;蛋白印迹法检测E-cadherin和N-cadherin蛋白表达;RT-qPCR检测circ_001680表达。结果钩吻碱或干扰circ_001680表达可降低RASFs的增殖、克隆形成数、划痕愈合率和侵袭细胞数及细胞中N-cadherin蛋白表达(P<0.05),而促进E-cadherin蛋白表达(P<0.05)。钩吻碱可降低RASFs中circ_001680的表达(P<0.05),过表达circ_001680减轻钩吻碱对RASFs增殖、迁移和侵袭的抑制作用。结论钩吻碱可抑制RASFs增殖、迁移和侵袭,其作用机制可能与下调circ_001680表达有关。

Gelsemine inhibits proliferation and invasion of rheumatoid arthritis synovial fibroblast through down-regulating circ_001680

Objective To investigate the effect of gelsemine on the proliferation,migration and invasion of rheumatoid arthritis endothelial cells(RASFs)and its possible mechanism.Methods RASFs were isolated and cultured.After RASFs were intervened with 50,100,200μg/mL gelsemine for 24 h or small interfering RNA or over-expression vector of circular RNA circ_001680 was transfected into RASFs,cell proliferation was measured by CCK-8 method and clone formation test,cell migration was detected by scratch test,cell invasion was examined by Transwell,the protein expression of E-cadherin and N-cadherin was detected by Western blot.The protein expression of circ_001680 was examined by RT-qPCR.Results Gelsemine or interference with circ_001680 expression reduced the A value,the number of clones,the healing rate of scratches and the number of invaded cells of RASFs and the protein expression of N-cadherin in cells(P<0.05),and promoted the protein expression of E-cadherin(P<0.05).Gelsemine reduced the expression of circ_001680 in RASFs(P<0.05),and the over-expression of circ_001680reduced the inhibitory effect of gelsemine on the proliferation,migration and invasion of RASFs.ConclusionsGelsemine may inhibit the proliferation,migration and invasion of RASFs,and its mechanism is potentially related to the down-regulation of circ_001680 expression.