Enrichment of Residual Tumor Cells in Bone Marrow Or Peripheral Blood Cells in a Neuroectodermal Tumor Model |
| |
Authors: | Bernhard Kremens Regina Wieland Renate Reuss Klaus Lennartz Werner Havers |
| |
Affiliation: | a Department of Pediatric Hematology-Oncology, University of Essen, Essen, Germanyb Department of Cell Biology, University of Essen, Essen, Germany |
| |
Abstract: | To enrich a small population of malignant cells contaminating human bone marrow or peripheral blood, a method of immunomagnetic depletion of the normal nucleated cells was developed. In a model using neuroectodermal cell lanes, contaminations between 0.1% and 1.4% of tumor cells could be increased by a factor of 7.5 (median; range, 3.8 to 18.0; n = 10). Because incubation with antibodies is restricted to the magnetic beads in this method, th cell population after removal of the beads has not been incubated with antibodies. The risk of unspecific staining of the tumor cells during the enrichment procedure is minimal. This simple method is therefore well suited to the study of the characteristics of a minimal residual disease population. |
| |
Keywords: | bone marrow enrichment of tumor cells neuroectodermal cell line peripheral blood residual disease |
本文献已被 InformaWorld 等数据库收录! |
|