蛹虫草菌丝体多糖与子实体多糖体外免疫调节活性的研究

目的 分析蛹虫草菌丝体多糖（mycelia polysaccharides of Cordyceps militaris，MPS）与蛹虫草子实体多糖（polysaccharides from fruiting body of Cordyceps militaris，FPS）的体外免疫活性。方法 以RAW264.7细胞株为受试对象，从细胞增殖、中性红吞噬能力、NO释放水平角度系统考察不同浓度的MPS与FPS的免疫调节活性，并分析其量化关系。结果 MPS与FPS均可明显促进RAW264.7细胞株的增殖、对中性红的吞噬能力和NO释放水平，且均呈现出浓度依赖性的特征。二者对RAW264.7细胞株增殖的促进作用无显著性差异，但在免疫调节最佳作用浓度方面却存在一定差异，MPS在100μg·mL^-1时表现出最好的细胞吞噬能力，而FPS在200μg·mL^-1时表现出最高的细胞吞噬能力。结论 MPS与FPS均具有显著的体外免疫活性。

Study on in Vitro Immune Activity of Polysaccharides from Mycelium and Fruiting Body of Cordyceps militaris

OBJECTIVE To analyze the in vitro immune activity of polysaccharides from mycelium and fruiting body of Cordyceps militaris.METHODS For investigating the immune activity of polysaccharide of Cordyceps militaris, RAW264.7 cell strain was chosen to supply with different concentration of polysaccharides from mycelium and fruiting body of Cordyceps militaris, and the cell proliferation index, cell''s phagocytosis ability of natural red and nitric oxide release level were analyzed, respectively. The quantitative relation of concentration of polysaccharide and the in vitro immune activity was also studied.RESULTS Both of polysaccharides from mycelium and fruiting body of Cordyceps militaris could show significantly promoting effects on RAW264.7 cell growth, phagocytosis ability of natural red and release of nitric oxide, which was positively associated with the concentration polysaccharide, and no significant difference were found between the cell growth promoting effects of different polysaccharides in the same dose, but the optimal concentration with best immune activity of polysaccharides from mycelium and polysaccharides from fruiting body of Cordyceps militaris were different.CONCLUSION Both of polysaccharides from mycelium and polysaccharides from fruiting body of Cordyceps militaris can show significantly in vitro immune activity.