花青素对小鼠巨噬泡沫细胞胆固醇外流的影响

目的：研究植物化学物质花青素对小鼠巨噬泡沫细胞胆固醇外流的影响，探讨花青素促进巨噬泡沫细胞胆固醇外流的分子机制。方法： 制备乙酰化低密度脂蛋白（AcLDL），以其负载小鼠腹腔巨噬细胞形成巨噬泡沫细胞，观察不同浓度花青素Cy-3-G和Pn-3-G对巨噬泡沫细胞胆固醇外流的影响以及调节胆固醇外流有关的基因过氧化物酶体增殖物激活受体-γ（PPAR-γ）表达的情况。结果：AcLDL可促进胆固醇在巨噬细胞中大量蓄积，造成泡沫细胞的形成。花青素Cy-3-G和Pn-3-G能引起巨噬泡沫细胞内胆固醇的大量外流，并且增加PPAR-γ基因的表达。结论：花青素Cy-3-G和Pn-3-G促进胆固醇外流的作用与其增加PPAR-γ基因的表达有关。

Effects of anthocyanin on cholesterol efflux from mouse peritoneal macrophage foam cells

AIM: To explore the effect of anthocyanin on cholesterol efflux and elucidate its possible molecular mechanism. METHODS: Mouse Peritoneal macrophages were loaded with 50 mg/L AcLDL to induce macrophage-derived foam cells. Cholesterol efflux from macrophage-derived foam cells induced by anthocyanin was determined by enzymatic methods. PPAR-γ mRNA and protein expression in macrophage-derived foam cells was assayed by quantitative PCR and western blotting. RESULTS: Anthocyanins had the capacity of promoting cholesterol efflux from mouse peritoneal macrophage-derived foam cells and increased PPAR-γ mRNA and protein expression. CONCLUSION: Anthocyain-induced cholesterol efflux may be related to its enhancing PPAR-γ mRNA and protein expression.