| 化学发光法检测梅毒螺旋体特异性抗体的方法学评价 |
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| 引用本文： | 郑和平,黄进梅,黄澍杰,李燕娃,蔡顺鑫,徐刚.化学发光法检测梅毒螺旋体特异性抗体的方法学评价[J].中国艾滋病性病,2008,14(6). |
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| 作者姓名： | 郑和平 黄进梅 黄澍杰 李燕娃 蔡顺鑫 徐刚 |
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| 作者单位： | 郑和平,黄进梅(广东省皮肤性病防治中心,广州,510500);黄澍杰(江门市皮肤医院,广东,江门,529000);李燕娃(佛山市皮肤病防治所,广东,佛山,528000);蔡顺鑫(韶关市皮肤病医院,广东,韶关,519000);徐刚(珠海市慢性病防治院,广东,珠海,512026) |
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| 摘 要： | 目的 评价化学发光法检测梅毒螺旋体特异性抗体的效果.方法 5家实验室分别用酶联免疫吸附试验(ELISA)、化学发光检测法(CLIA)和梅毒螺旋体明胶颗粒凝胶试验(TPPA)进行临床标本的双盲检测.以TPPA结果为标准,分析其敏感性和特异性.结果 共计检测1 525例血清样品,其中TPPA确证阳性308例,阳性率为20.2%.以TPPA作参照,发光法敏感性为100%(308/308),特异性为99.3%(1 208/1 217),阳性结果预测值(PPV)为97.2%(308/317),阴性结果预测值(NPV)为100%(1 208/1 208);5种ELISA试剂总体敏感性为98.1%(302/308),特异性为97.9%(1 192/1 217),PPV为92.4%(302/327),NPV为99.5%(1 192/1 198).结论 CLIA法具有敏感性高和特异性强的特点,适合于临床实验室进行梅毒的筛查.
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| 关 键 词： | 梅毒 抗体 化学发光 评价 |
Evaluation of chemiluminescence immunoassay (CLIA) for detecting the antibody to Treponema palledum |
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| ZHENG He-ping,HUANG Jin-mei,HUANG Shu-jie,et al..Evaluation of chemiluminescence immunoassay (CLIA) for detecting the antibody to Treponema palledum[J].Chinese JOurnal of Aids & STD,2008,14(6). |
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| Authors: | ZHENG He-ping HUANG Jin-mei HUANG Shu-jie |
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| Institution: | ZHENG He-ping,HUANG Jin-mei,HUANG Shu-jie,et al.(Guangdong Provincial Centers for Skin Diseases , STIs Control , Prevention,Guangzhou.Guangdong 510500,China) |
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| Abstract: | Objective To evaluate the effect of chemiluminescence immunoassay (CLIA) for detecting the antibody to Treponema palledum.Methods The evaluation was carried out by double blind experiments with ELISA,CLIA and TPPA in five different laboratories.Compared with the results of TPPA as a reference,the specificity and sensitivity were analyzed. Results Out of 1 525 sera detected,308 were positive by TPPA,accounting for 20.2%.Compared with the results of TPPA,the sensitivity,specificity,positive predictive value (... |
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| Keywords: | Syphilis Antibody Chemiluminescence Immunoassay Evaluation |
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