| 去势雌性大鼠海马区Tau蛋白Alzheimer样磷酸化的变化 |
| |
| 引用本文： | 代小燕,刘义,王泽华,张蕲,王建枝.去势雌性大鼠海马区Tau蛋白Alzheimer样磷酸化的变化[J].生殖医学杂志,2005,14(2):83-85. |
| |
| 作者姓名： | 代小燕 刘义 王泽华 张蕲 王建枝 |
| |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院妇产科,武汉,430022
2. 华中科技大学同济医学院病理生理教研室,武汉,430030 |
| |
| 基金项目： | 湖北省卫生厅科研基金(2003JXB034) |
| |
| 摘 要： | 目的探讨去势雌性大鼠海马区Tau蛋白Alzheimer样磷酸化的变化。方法建立大鼠双侧卵巢切除(OVX)模型,分别于术后1、2、3、4和8周取大鼠海马组织,WesternBlot检测Tau蛋白的磷酸化程度。结果与假手术组相比,OVX组去势后4和8周在海马区的PHF1位点异常磷酸化Tau蛋白显著升高(P<0.01),而相同时间点和相同区域的Tau1位点非磷酸化Tau蛋白的水平显著降低(P<0.01)。结论雌激素缺乏可导致雌性大鼠海马区Tau蛋白Alzheimer样过度磷酸化。
|
| 关 键 词： | 雌激素 阿尔茨海默病 Tau蛋白 OVX大鼠 海马 |
| 文章编号： | 1004-3845(2005)02-0083-03 |
| 修稿时间： | 2004年9月27日 |
The change of Alzheimer-like phosphorylation of Tau proteins in hippocampus of ovariectimized rats |
| |
| DAI Xiao-yan,LIU Yi,WANG Ze-hua,ZHANG Qi,WANG Jian-zhi.The change of Alzheimer-like phosphorylation of Tau proteins in hippocampus of ovariectimized rats[J].Journal of Reproductive Medicine,2005,14(2):83-85. |
| |
| Authors: | DAI Xiao-yan LIU Yi WANG Ze-hua ZHANG Qi WANG Jian-zhi |
| |
| Institution: | DAI Xiao-yan~1,LIU Yi~1,WANG Ze-hua~1,ZHANG Qi~2,WANG Jian-zhi~21.Department of Obstetrics & Gynecology,Union Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 4300222.Department of Pathophysiology,Neuroscience Institute,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430030 |
| |
| Abstract: | Objective: To explore the changes of Alzheimer-like phosphorylation of Tau proteins in hippocampus of ovariectimized (OVX) Sprague-Dawley rats.Methods: The OVX rats were used as a model. Hippocampus of OVX rats was removed from the rat brains on 1w, 2w, 3w, 4w and 8w after the ovariectomy, respectively, and the phosphorylation of tau protein was measured by Western blotting.Results: The levels of phosphorylated Tau proteins at PHF-1 epitope were elevated in OVX rat brain hippocampus on 4 w and 8 w after the ovariectomy, when compared with those in sham-OVX rats (P<(0.01).) On the other hand, the levels of Tau non-phosphorylated proteins at Tau-1 site were decreased in the same brain regions at the same time point examined (P<0.01).Conclusion: The absence of estrogen may induce Alzheimer-like hyperphosphorylation of Tau protein in the OVX rat brain hippocampus. |
| |
| Keywords: | Estrogen Alzheimer disease Tau proteins Ovariectimized rat Hippocampus |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
