| 体外循环对大鼠血脑屏障及紧密连接蛋白表达的影响 |
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| 引用本文： | 曹惠鹃,孙莹杰,张铁铮,周锦,姚婧.体外循环对大鼠血脑屏障及紧密连接蛋白表达的影响[J].中国体外循环杂志,2011,9(2):102-104,128. |
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| 作者姓名： | 曹惠鹃 孙莹杰 张铁铮 周锦 姚婧 |
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| 作者单位： | 沈阳军区总医院麻醉科,沈阳,110016 |
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| 摘 要： | 目的探讨体外循环（CPB）对大鼠血脑屏障（BBB）功能及脑血管内皮紧密连接蛋白表达的影响。方法 24只成年雄性SD大鼠,随机分为假手术组（S组）和体外循环组（C组）。S组进行相应的麻醉和手术操作但不进行CPB,C组进行CPB 60 min并继续观察2 h。每组随机取6只测脑组织伊文思蓝（EB）含量,另6只做脑组织含水量测定、紧密连接蛋白Oc-cludin和ZO-1的表达以及大鼠BBB超微结构的观察。结果与S组比较,C组大鼠海马区脑组织EB含量和含水量升高,差异均有统计学意义（P〈0.05）。与S组比较,C组大鼠脑血管内皮紧密连接蛋白Occludin和ZO-1表达水平下降,差异均有统计学意义（P〈0.05）。S组BBB基膜均匀一致,完整性良好,内皮细胞间的紧密连接完整;C组基底膜变厚,微血管腔狭窄,内皮细胞间紧密连接不清楚。结论 CPB使大鼠BBB的结构发生变化、通透性增加,其机制可能与大鼠脑血管内皮紧密连接蛋白改变有关。
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| 关 键 词： | 体外循环 紧密连接蛋白 血脑屏障 |
Effects of cardiopulmonary bypass on blood-brain barrier and the expression of tight junction protein in rats |
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| Cao Hui-juan,Sun Ying-jie,Zhang Tie-zheng,Zhou Jin,Yao Jing.Effects of cardiopulmonary bypass on blood-brain barrier and the expression of tight junction protein in rats[J].Chinese Journal of Extracorporeal Circulation,2011,9(2):102-104,128. |
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| Authors: | Cao Hui-juan Sun Ying-jie Zhang Tie-zheng Zhou Jin Yao Jing |
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| Institution: | Cao Hui-juan,Sun Ying-jie,Zhang Tie-zheng,Zhou Jin,Yao JingDepartment of Anesthesiology of the General Hospital of ShenYang Military Region,Shenyang 110016,China |
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| Abstract: | OBJECTIVE To investigate the effects of cardiopulmonary bypass(CPB) on blood-brain barrier(BBB) and the expression of tight junction(TJ) protein of cerebral vascular endothelium in rat model.METHODS 24 adult male Sprague-Dawley rats were randomly divided into two groups: group S served as shame-operated group without CPB;the rats of group C underwent 60 minutes of CPB and were observed 2 hours later.The Evans blue content of brain tissue was measured in 6 rats of each group,the water content of brain tissue... |
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| Keywords: | Cardiopulmonary bypass Tight junction Blood-brain barrier |
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