| HER2基因RNA干扰质粒对SK-BR-3细胞增殖及凋亡的影响 |
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| 引用本文： | 韩冬,徐煌,李文浩,张成文.HER2基因RNA干扰质粒对SK-BR-3细胞增殖及凋亡的影响[J].中国实验诊断学,2011,15(5):802-805. |
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| 作者姓名： | 韩冬 徐煌 李文浩 张成文 |
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| 作者单位： | 嘉兴学院医学院,浙江,嘉兴314001 |
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| 摘 要： | 目的探讨HER2基因高效RNA干扰质粒转染到SK-BR-3乳腺癌细胞后,对细胞的增殖及其凋亡的影响。方法采用脂质体转染法将针对HER2基因的高效RNA干扰质粒转染至乳腺癌细胞SK-BR-3中,通过细胞计数、MTT比色法、流式细胞术和Western blot检测PCNA增殖细胞核抗原,分析检测其对SK-BR-3细胞增殖、凋亡的影响。结果 MTT比色测定显示,HER2-shRNA2干扰质粒转染到SK-BR-3细胞后在48 h后现抑制,与空白对照组比抑制率分别为48 h 16.53%7、2h 39.03%9、6h 65.47%。流式细胞分析HER2-shRNA组细胞在96 h凋亡率达17.36%,明显高于阴性对照组的1.41%和空白对照组的1.25%（P〈0.05）。结论将RNA干扰质粒HER2-shRNA2转染至SK-BR-3细胞中,可特异性地抑制SK-BR-3细胞的增殖并诱导其凋亡,为乳腺癌的靶向治疗奠定了基础。
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| 关 键 词： | HER2基因 RNA干扰 乳腺癌 |
RNAi silencing of HER2 gene induces apoptosis and antiproliferative effect of SK-BR-3 cell line |
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| HAN Dong,XU Huang,LI Wen-hao,et al..RNAi silencing of HER2 gene induces apoptosis and antiproliferative effect of SK-BR-3 cell line[J].Chinese Journal of Laboratory Diagnosis,2011,15(5):802-805. |
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| Authors: | HAN Dong XU Huang LI Wen-hao |
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| Institution: | HAN Dong,XU Huang,LI Wen-hao,et al.(Department of Biochemistry,School of Medicine,Jiaxing College,Jiaxing 314001,China) |
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| Abstract: | Objective To examine the biologic effects of HER2 gene silencing on SKBR3 cells through the transfection of HER2-specific shRNA expression vector.Methods SK-BR-3 breast cancer cells overexpressing the HER2 gene were transfected with HER2-shRNA2 vector using Lipofectamine2000.After transfection the antiproliferative effect of HER2-shRNA2 was detected by MTT assay and apoptosis percentage was detected by flow cytometry.The expressions of proliferating cell nuclear antigen(PCNA) were evaluated by Western blot ... |
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| Keywords: | HER2 gene RNA interference breast cancer |
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