| G250基因沉默诱导肾癌786-0细胞凋亡 |
| |
| 引用本文： | 赵俊峰,郑少斌,赵善超,姜耀东,肖耀军,杨旭凯.G250基因沉默诱导肾癌786-0细胞凋亡[J].中国实验诊断学,2011,15(4):579-582. |
| |
| 作者姓名： | 赵俊峰 郑少斌 赵善超 姜耀东 肖耀军 杨旭凯 |
| |
| 作者单位： | 1. 河南省中医院、河南中医学院第二附属医院,泌尿外科,河南,郑州450002
2. 南方医科大学南方医院,泌尿外科,广东,广州510515 |
| |
| 摘 要： | 目的观察沉默G250基因对肾癌786-0细胞凋亡的影响。方法构建靶向G250的siRNA表达质粒pshRNA-G250并转染肾癌786-0细胞,TUNEL法和流式细胞仪检测G250基因沉默后肾癌786-0细胞（命名786-0/si-G250-b）的凋亡率,以转染空质粒的细胞（命名786-0/si-G250-0）为对照。结果 TUNEL法显示786-0/si-G250-b和786-0/si-G250-0细胞凋亡率分别为（21.537±3.552）%、（6.590±1.167）%,（t=6.925,P=0.002）,流式细胞仪检测示细胞凋亡率分别为（33.033±2.914）%（、9.133±1.115）%,（t=13.266,P=0.000）。结论沉默G250基因的表达可诱导肾癌786-0细胞的明显凋亡。
|
| 关 键 词： | G250基因 凋亡 肿瘤相关抗原 RNA干扰 肾细胞癌 |
Apoptosis of renal cell carcinoma 786-0 cells induced by G250 gene silencing |
| |
| ZHAO Jun-feng,ZHENG Shao-bin,ZHAO Shan-chao,et al..Apoptosis of renal cell carcinoma 786-0 cells induced by G250 gene silencing[J].Chinese Journal of Laboratory Diagnosis,2011,15(4):579-582. |
| |
| Authors: | ZHAO Jun-feng ZHENG Shao-bin ZHAO Shan-chao |
| |
| Institution: | ZHAO Jun-feng1,ZHENG Shao-bin2*,ZHAO Shan-chao2,et al.(1.Department of Urology,Second affiliated Hospital of Henan Traditional Chinese Medicine University,Henan Zhengzhou 450002,China,2.Department of Urology,Nanfang Hospital,Southern Medical University,Guangdong Guangzhou 510515,China) |
| |
| Abstract: | Objective To investigate the effects of G250 gene silencing by siRNA on apoptosis of renal cell carcinoma 786-0 cell.Methods The eukaryotic expression vector named pshRNA-G250 of small interfering RNA(siRNA) targeting G250 gene was constructed and transfected into renal cell carcinoma 786-0 cells.TUNEL and flow cytometry(FCM) assay were performed to test the rate of apoptosis of 786-0 cells after G250 gene silencing(named 786-0/si-G250-b).and one that empty plasmid was transfected into(named 786-0/si-G250-0... |
| |
| Keywords: | G250 gene apoptosis tumor associate antigen RNA interference renal cell carcinoma |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
