Observations of the Number of Available c, D, and E Antigen Sites on Red Cells |
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| Authors: | Dr. N. C. HUGHES-JONES BRIGITTE GARDNER and Dr. P. J. LINCOLN |
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| Affiliation: | Medical Research Council's Experimental Haematology Unit, St. Mary's Hospital Medical School, and the Department of Forensic Medicine, The London Hospital, London |
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| Abstract: | Abstract. The number of available antigen sites within the Rh system were estimated using trace-labelled antibodies. The results were as follows, given as the number of sites per red cell: c-antigen: on cc cells, 70,000–85,000: on Cc, 37,000–53,000; D sites on cells of phenotype -D-: 110,000–202,000; E-antigen sites showed considerable heterogeneity depending on phenotype as well as source of anti-E, and estimates varied between 450 and 25,600; e-antigen: on eE cells, 13,400 and 14,500: on ee cells, 18,200 and 24,400.
The average equilibrium constants of the antibodies were: anti-E, 4 × 108 1/mol; anti-e, 2.5 × 108 1/mol; anti-c, 3.2 × 107 and 5.6 × 107 1/mol. |
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