MMP-9、bFGF在子宫内膜异位症患者血清与腹腔液中表达的相关性研究

目的通过考察子宫内膜异位症患者、非子宫内膜异位症患者腹腔液及血清中基质金属蛋白酶-9(MMP-9)、碱性成纤维细胞生长因子(bFGF)含量的变化,探讨两者在子宫内膜异位症发病机制的意义。方法采用ELISA法分别检测45例子宫内膜异位症患者(Ⅰ～Ⅱ期23例,Ⅲ～Ⅳ期22例)和20例非子宫内膜异位症患者腹腔液及血清中MMP-9、bFGF的含量,同时进行相关性分析。结果内异症组腹腔液中MMP-9、bFGF含量明显高于对照组,差异有统计学意义(P<0.01);Ⅲ～Ⅳ期内异症组患者腹腔液中MMP-9、bFGF含量均明显高于Ⅰ～Ⅱ期内异症组,差异均有统计学意义(P<0.01);内异症组血清中MMP-9、bFGF含量明显高于对照组,差异有统计学意义(P<0.01);Ⅲ～Ⅳ期内异症组血清中MMP-9、bFGF含量均明显高于Ⅰ～Ⅱ期内异症组,差异均有统计学意义(P<0.01)。结论MMP-9与bFGF在子宫内膜异位症发生、发展、变化过程中发挥着重要的协同作用。MMP-9、bFGF在子宫内膜异位症患者体中高表达,为异位内膜的"侵袭"与"血管生成"提供理论依据。

Studies on correlation between the content of MMP-9 and bFGF in serum and peritoneal fluid of patients with endometriosis

Objective To detect MMP-9 and bFGF concentration variation in serum and peritoneal fluid of patients with endometriosis and non-endometriosis,discuss the both in the pathogenesis of endometriosis meaning. Methods By using ELISA method, detected respectively MMP-9 and bFGF content from 45 patients with endometriosis （23 patients in stages Ⅰ and Ⅱ,22 patients in stages Ⅲ and Ⅳ ） and 20 women without endometriosis. At the same time,we analyzed the correlation between them. Results The peritoneal fluid MMP-9 and bFGF concentrations in endometriosis group were sig- nificantly higher than those in control group, differences were statistically significant （P 〈 0.01） ;the peritoneal fluid MMP- 9 and bFGF concentrations in patients with stage m and Ⅳ endometriosis group were significantly higher than those in patients with stage Ⅰ and Ⅱ endometriosis group, differences were statistically significant（P 〈 0.01 ）;the serum MMP-9 and bFGF concentrations in endometriosis group were significantly higher than that those in control group,differences were statistically significant（P 〈 0.01 ） ;The serum MMP-9 and bFGF concentrations in patients with stage Ⅲ and Ⅳ endometrio- sis group were significantly higher than that in patients with stage Ⅰ and Ⅱ endometriosis group,differences were statisti- cally significant（P 〈 0.01 ）. Conclusion In endometriosis occurrence,development,change process, MMP-9 and bFGF played an important synergy. The high content of MMP-9 and bFGF in patients with endometriosis provided a theoretical basis which was the ectopic endometrium ＂aggression＂ and ＂angiogenesis＂.