| COM.TEC型血细胞分离机采集外周血干细胞效果观察 |
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| 引用本文： | 何静,陈方祥,王劲,滕方,曾永芳.COM.TEC型血细胞分离机采集外周血干细胞效果观察[J].重庆医学,2009,38(12). |
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| 作者姓名： | 何静 陈方祥 王劲 滕方 曾永芳 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所输血科,重庆,400042;第三军医大学大坪医院野战外科研究所血液科,重庆,400042 |
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| 摘 要： | 目的 观察COM.TEC型血细胞分离机MNC程序采集外周血造血干细胞效果.方法 用COM.TEC型血细胞分离机MNC程序采集16例血液系统肿瘤患者外周血干细胞,患者采用化疗、粒细胞集落刺激因子动员,采集的干细胞用流式细胞仪检测CD34+细胞数.共采集32次,每例采集2次,每次处理抗凝全血(9 158±2 166)mL,抗凝剂(919.78±230.60)mL,时间(178.1±38.0)min.结果 采集1次CD34+细胞数为(1.62±0.32)×106/kg,MNC采集效率为(81.25±14.57)%,单采后患者血小板(plt)、血红蛋白(Hb)、红细胞比容(Hct)分别下降13.0%、7.2%、9.1%,与采集前比较,差异无统计学意义(P＞0.05).采集过程中未发生严重不良反应.结论 COM.TEC型血细胞分离机MNC程序能采集到血小板含量低、产量高而有效的自体CD34+细胞,采集方法可靠,可以满足临床治疗要求.
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| 关 键 词： | 血细胞分离机 造血干细胞/采集 CD34+细胞 外周血 |
The efficiency of COM.TEC blood cell separator in collecting peripheral blood stem cells |
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| HE Jing,CHEN fang-xiang,WANG Jing,et al..The efficiency of COM.TEC blood cell separator in collecting peripheral blood stem cells[J].Chongqing Medical Journal,2009,38(12). |
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| Authors: | HE Jing CHEN fang-xiang WANG Jing |
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| Institution: | HE Jing1,CHEN fang-xiang1,WANG Jing2,et al.(1.Department of Blood Transfusion,2.Department of Hemology,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To observe the efficiency of Fresenius COM.TEC MNC process in collection of peripheral hematopoietic stem cell.Methods The Fresenius COM.TEC blood cell separator was utilized to collect hematopoietic stem cell from 16 patients which suffer from neoplastic hematologic disorder.The patients were treated with chemo add G-CSF arouse.The number of CD34+cell antigent presentation were detected by flow cytometry.With 16 patient 32 times collect and each patient 2 times.Disposal anticoagulation blood(9 15... |
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| Keywords: | blood cell separator hematopoietic stem cell/collect CD34 peripheral blood |
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