体外三维抗血管生成药物筛选模型的建立

目的:建立一种新的用于筛选具有抗血管生成作用的体外血管生成模型。方法:人脐静脉的内皮细胞(HUVECs)在三维胶原纤维系统中培养，制备成具有毛细血管样网状结构模型；3种酪氨酸酶抑制剂genistein，tyrphostin A23和lavendustin C被分别加入到模型中，观察它们对血管形成的影响。结果:在不加入任何生长因子的条件下，HUVECs能迅速重组形成毛细血管样网状结构；3种酪氨酸酶抑制剂均对血管形成有抑制作用。结论该模型具有操作简单、可重复性好、易量化的特点。是一种在体外筛选血管生成抑制剂的理想模型。

Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection]

OBJECTIVE: To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances. METHODS: Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro, on which the antiangiogenic effects of genistein, tyrphostin A23 and lavendustin C were assessed. RESULTS: HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture, and was easily reproducible. Tyrosine kinase inhibitors, genistein, tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation. CONCLUSION: This new human angiogenesis model has proved to be simple, responsive, and reproducible for testing potential angiogenic inhibitors, and is suitable for screening anti-angiogenic drugs.