| 石斛总DNA的提取及鉴定 |
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| 引用本文： | 彭锐,宋洪元,李泉森,王宇.石斛总DNA的提取及鉴定[J].中国中药杂志,2003,28(12):1129-1131. |
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| 作者姓名： | 彭锐 宋洪元 李泉森 王宇 |
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| 作者单位： | 1. 重庆市中药研究院,重庆,400065
2. 西南农业大学园艺系,重庆,400716 |
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| 基金项目： | 重庆市科委应用基础课题 (2 0 0 0 672 12 ) |
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| 摘 要： | 目的 :探讨不同DNA提取方法对石斛属DNA质量及分子标记结果的影响。方法 :对提取的DNA进行紫外光谱分析、总DNA电泳及RAPD分析。结果 :不同方法提取的DNA质量及产率存在显著差异 ,对RAPD结果影响很大。结论 :石斛属植物在DNA提取前加入无CTAB缓冲液抽提多糖等杂质 ,可获得较高质量的DNA。
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| 关 键 词： | 石斛属 总DNA RAPD 电泳 紫外光谱 |
| 文章编号： | 1001-5302(2003)12-1128-03 |
| 收稿时间： | 2002/11/8 0:00:00 |
| 修稿时间： | 2002年11月8日 |
Extraction and Characterization of Total DNA from Dendrobium |
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| PENG Rui ;SONG Hong-yuan ;LI Quan-sen ;WANG Yu.Extraction and Characterization of Total DNA from Dendrobium[J].China Journal of Chinese Materia Medica,2003,28(12):1129-1131. |
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| Authors: | PENG Rui ;SONG Hong-yuan ;LI Quan-sen ;WANG Yu |
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| Institution: | Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. wangyhl1971@yahoo.com |
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| Abstract: | Objective: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR. Method: the electrophoresis of total DNA ,UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods. Result: The DNA concentration and yields were different,which greatly influenced the result of RAPD-PCR. Conclusion: The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated. |
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| Keywords: | Dendrobium total DNA RAPD electrophoresis UV spectrophotometry |
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