脑热清对内毒素性发热家兔血管内皮细胞分泌功能的影响

目的从血管内皮细胞分泌功能探讨脑热清（NRQ）口服液对内毒素性发热家兔的作用。方法复制内毒素性发热家免模型，观察NRQ对家免体温的影响，采用放免法测定血浆血栓素B2（TXB2）、6-酮-前列腺素F1α（6-keto-PGF1α）以及内皮素（endothelin，ET）的含量，用发色底物法测定血浆组织纤溶酶原激活物（t-PA）和组织纤溶酶原激活物抑制剂（PAI）的活性。结果NRQ＋内毒素组的△T（0．69±0．07）℃]、TRI2（4．85±0．57）、ET（197．96±39．11）ng／L]和TXB2（177．35±77．30）ng／L]的含量以及PAI（0．84±0．01）AU／ml]的活性明显低于内毒素组的△T（1．31±0．13）℃]、TRI2（8．44±0．98）、ET（250.80±40．99）ng／L]和TXB2（279.64±83.74）ng／L]的含量以及PAI（0．86±0．01）AU／ml]的活性，两者比较差异有显著性（P〈0．05，P〈0．01）；而6-keto-PGF1α（986．70±327．36）ng／L]的含量和t-PA（0．25±0．02）IU／ml]的活性明显高于内毒素组6-keto-PGF1α（507．81±170．01）ng／L]的含量和t-PA（0．21±0．02）IU／ml]的活性，两者比较差异亦有显著性（P〈0．01）。结论NRQ可能通过改善发热家兔血管内皮细胞的分泌功能，起到扩张血管，加快体表散热，从而对内毒素性发热家兔的解热效应起到一定的整合作用。

Effects of Naoreqing on Secretive Function of Vaso-endothelial Cells in Rabbits with Endotoxic Fever

Objective: To observe the antifebrile effect of Naoreqing (NRQ) oral liquid on secretive function of vaso-endothelial cells in rabbits with endotoxic fever. METHODS: Endotoxic fever rabbit model was duplicated to observe the effects of NRQ on body temperature, blood levels of thromboxane B2 (TXB2), 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) and endothelin (ET) using radioimmunoassay, as well as activity of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) in plasma by chromophoric substrate assay. RESULTS: Comparisons of various indexes between the two groups showed significantly difference, i.e. the maximal increment of body temperature: 0.69 +/- 0.07 degrees C vs 1.31 +/- 0.13 degrees C (the NRQ treated group vs the untreated model group, the same hereafter); 2h thermal response index TRI2 4.85 +/- 0.57 vs 8.44 +/- 0.98; plasma ET content 197.96 +/- 39.11 ng/L vs 250.80 +/- 40.99 ng/L; TXB2 content 177.35 +/- 77.30 ng/L vs 279.64 +/- 83.74 ng/L; activity of PAI 0.84 +/- 0.01AU/ml vs 0.86 +/- 0.01 AU/ml; plasma 6-keto-PGF1alpha content 986.70 +/- 327.36 ng/L vs 507.81 +/- 170.01 ng/L; activity of t-PA 0.25 +/- 0.02 IU/ml vs 0.21 +/- 0.02 IU/ml (P < 0.05, P < 0.01). CONCLUSION: NRQ may improve secretive function of vaso-endothelial cells to dilate blood vessel and quicken heat dissipation through body surface, so as to play an integral antipyretic effect in rabbits with endotoxic fever.