| 共表达红光荧光蛋白及发夹RNA载体的构建及鉴定 |
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| 引用本文： | 魏映辉,吴秀丽,万敏,于永利,王丽颖.共表达红光荧光蛋白及发夹RNA载体的构建及鉴定[J].吉林大学学报(医学版),2005,31(2):163-165. |
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| 作者姓名： | 魏映辉 吴秀丽 万敏 于永利 王丽颖 |
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| 作者单位： | 1. 吉林大学基础医学院分子生物学教研室，吉林 长春130021；2. 吉林大学基础医学院免疫学教研室，吉林 长春130021 |
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| 摘 要： | 目的：构建共表达红光荧光蛋白及短发夹状RNA的重组真核载体。
方法：利用亚克隆、T-A克隆和PCR技术构建pcDNA3.0/DsRed-U6-shGFR及pcDNA3.0/DsRed-U6重组载体。
结果：成功构建上述两种真核重组表达载体。
结论：这种共表达载体的构建为进一步利用RNAi技术研究真核细胞基因功能奠定了基础。
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| 关 键 词： | 发光蛋白质类 质粒 |
| 文章编号： | 1671-587X(2005)02-0163-03 |
| 收稿时间： | 2004-11-08 |
| 修稿时间： | 2004年11月8日 |
Construction and identification of vectors encoding both red fluorescent protein and shRNA |
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| WEI Ying-hui,WU Xiu-li,WAN Min,YU Yong-Li,WANG Li-ying.Construction and identification of vectors encoding both red fluorescent protein and shRNA[J].Journal of Jilin University: Med Ed,2005,31(2):163-165. |
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| Authors: | WEI Ying-hui WU Xiu-li WAN Min YU Yong-Li WANG Li-ying |
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| Institution: | 1. Department of Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;2. Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China |
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| Abstract: | Objective To construct the recombinant vectors that coexpress red fluorescent protein and small hairpin RNA (shRNA). Methods The subcloning, T-A cloning, and PCR technique were used to construct the recombinant vectors,named pcDNA3.0/DsRed-U6-shGFP and pcDNA3.0/DsRed-U6. Results The recombinant expression vectors mentioned above were successfully constructed.Conclusion The successful construction of recombinant vectors can establish a feasibility to further explore the functions of specific genes in eukaryotic cells with RNAi technique |
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| Keywords: | short hairpin RNAs luminescent proteins plasmids |
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