| 高效液相色谱法测定复方吴茱萸碱纳米乳的含量 |
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| 引用本文： | 柳珊,谭群友,廖红,王红,张景勍. 高效液相色谱法测定复方吴茱萸碱纳米乳的含量[J]. 中国医院药学杂志, 2012, 0(4): 244-246 |
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| 作者姓名： | 柳珊 谭群友 廖红 王红 张景勍 |
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| 作者单位： | 重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室;第三军医大学大坪医院野战外科研究所胸外科 |
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| 基金项目： | 教育部博士点基金资助项目(编号:20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助,编号:KJ090308) |
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| 摘 要： | 目的:建立高效液相色谱法测定复方吴茱萸碱纳米乳制剂中吴茱萸碱的含量。方法:通过有机溶剂法破乳,采用高效液相色谱法在225 nm波长处测定峰面积,测定复方吴茱萸碱纳米乳中吴茱萸碱的含量。结果:吴茱萸碱在1.25～10μg.mL-1范围内与峰面积呈良好的线性关系;回归方程为A=122 583C-33 922(r=0.999 7);平均回收率为99.96%,RSD为0.31%(n=9);日内及日间精密度的RSD均小于1%(n=5)。结论:建立的高效液相色谱法准确度高、专属性好,可用于吴茱萸碱复方纳米乳中吴茱萸碱的含量测定。
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| 关 键 词： | 吴茱萸碱 纳米乳 含量测定 高效液相色谱法 |
Content determination of evodiamine nanoemulsion by HPLC |
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| LIU Shan,TAN Qun-you,LIAO Hong,WANG Hong,ZHANG Jing-. Content determination of evodiamine nanoemulsion by HPLC[J]. Chinese Journal of Hospital Pharmacy, 2012, 0(4): 244-246 |
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| Authors: | LIU Shan TAN Qun-you LIAO Hong WANG Hong ZHANG Jing- |
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| Affiliation: | qing1(1.Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical&Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,China;2.Department of Thoracic Surgery,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | OBJECTIVE To establish a method for content determination of evodiamine in the nanoemulsion by HPLC.METHODS Ethanol was used for the emulsion breaking and HPLC was used for the content determination of the nanoemulsion at wavelength 225 nm.RESULTS Evodiamine showed a good linear relationship with the area in the range of 1.25-10 μg·mL-1.The regression equation was A=122 583C-33 922(r=0.999 7);the average recovery was 99.96% and RSD was 0.31%(n=9);RSD of the intra-and inter-day precision were lower than 1%(n=5).CONCLUSION The method is suitable for the determination of the content of evodiamine nanoemulsion. |
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| Keywords: | evodiamine nanoemulsion content determination HPLC |
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