| 葡萄内酯大鼠血浆蛋白结合率的测定 |
| |
| 引用本文： | 袁金斌,李明,陈海芳,刘莹莹,张瑜,杨武亮. 葡萄内酯大鼠血浆蛋白结合率的测定[J]. 中国医院药学杂志, 2012, 0(2): 94-96 |
| |
| 作者姓名： | 袁金斌 李明 陈海芳 刘莹莹 张瑜 杨武亮 |
| |
| 作者单位： | 江西中医学院现代中药制剂教育部重点实验室 |
| |
| 基金项目： | 科技部“重大新药创制”科技重大专项(编号:2009ZX09103-350);国家自然科学基金(编号:30660230);江西省卫生厅课题(编号:2009A057) |
| |
| 摘 要： | 目的:建立大鼠血浆中葡萄内酯的检测方法,测定葡萄内酯与大鼠血浆蛋白的结合率。方法:采用平衡透析法研究葡萄内酯在大鼠体内与血浆蛋白结合率。利用醋酸乙酯萃取富集袋内外样品中的葡萄内酯,并用HPLC法测定其浓度,进而求出血浆蛋白结合率。结果:在低、中、高3种浓度下,葡萄内酯的血浆蛋白结合率分别为100.0%、(99.7±0.6)%、(99.1±0.8)%。结论:葡萄内酯溶液与大鼠血浆蛋白属高度结合。
|
| 关 键 词： | 葡萄内酯 血浆蛋白结合率 平衡透析法 高效液相色谱法 |
Determination of the protein binding rates of aurapetne with rats plasma |
| |
| YUAN Jin-bin,LI Ming,CHEN Hai-fang,LIU Ying-ying,ZHANG Yu,YANG Wu-liang. Determination of the protein binding rates of aurapetne with rats plasma[J]. Chinese Journal of Hospital Pharmacy, 2012, 0(2): 94-96 |
| |
| Authors: | YUAN Jin-bin LI Ming CHEN Hai-fang LIU Ying-ying ZHANG Yu YANG Wu-liang |
| |
| Affiliation: | (Key Laboratory of Modern Preparation of TCM,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Jiangxi Nanchang,330004) |
| |
| Abstract: | OBJECTIVE To establish a chromatographic method of auraptene in rats plasma,and determine the binding rate of auraptene with rats plasma protein.METHODS The binding rate of auraptene with rats plasma was investigated with equilibrum dialysis method.Auraptene in the dialysate was concentrated with acetic ether,and its contents were determined by HPLC,then the binding rates were calculated.RESULTS The binding rate of auraptene with rats plasma protein was 100.0%,(99.7±0.6)%,and(99.1±0.8)%,respectively.CONCLUSION The binding rates of auraptene with rat plasma protein belong to the high-rate combination. |
| |
| Keywords: | auraptene protein binding rate equilibrium dialysis method HPLC |
| 本文献已被 CNKI 等数据库收录！ |
|
