COX-2基因及产物在人类喉癌组织中的表达

目的:研究COX-2在喉鳞状细胞癌中的表达,探讨其与喉癌的生长、浸润及转移的关系.方法:应用半定量RT-PCR方法对标本中COX-2 mRNA的表达进行检测,应用免疫组织化学方法对标本中COX-2蛋白的表达进行检测,并对COX-2的表达情况与疾病生物学特点的关系进行研究.结果:COX-2 mRNA及蛋白在喉鳞状细胞癌组织中高表达,与在声带息肉组织中的表达差异有统计学意义(P<0.01),且随肿瘤的进展COX-2的表达强度增加.结论:COX-2是较为有效的生物学指标,指示喉鳞状细胞癌的发生和发展.同时应用RT-PCR方法及免疫组织化学方法检测喉癌标本,既保证了灵敏度又兼顾了特异度.

The expression of COX-2 mRNA and protein in human laryngeal squamons cell carcinoma

Objective:To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade,clinical stage and lymphoma metastasis.Method:By using emi-quantify RT-PCR and Immunohistochemistry,the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.Result:Compared with the polyp of vocal cord tissue,the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased(P<0.01).The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.Conclusion:COX-2 is high expressed in human laryngeal squamous cell carcinoma,and it may play an significant role in the growth,invasion and metastasis of human laryngeal squamous cell carcinoma.A combination of emi-quantify RT-PCR and immunohistochemisty is sensitive and specific for laryngeal neoplasms detection.