| 膀胱癌多发或复发的克隆起源 |
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| 引用本文： | 叶定伟,李慧,Peter Murphy.膀胱癌多发或复发的克隆起源[J].中华泌尿外科杂志,2000,21(5):280-281. |
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| 作者姓名： | 叶定伟 李慧 Peter Murphy |
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| 作者单位： | 第二军医大学长海医院泌尿外科(叶定伟!200433上海，孙颖浩!200433上海，钱松溪!200433上海，郑家富!200433上海，马永江!200433上海)，第二军医大学长海医院内分泌科(李慧!200433上海)，美国BaylorCollegeofMedicine(Peter Murphy) |
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| 摘 要： | 目的 探讨人膀胱移行上皮癌多发性、复发性的细胞克隆起源。方法 采用ｐ５３基因突变的ＰＣＲ－ＳＳＣＰ分析及ＤＮＡ测序检测６例患者１４个表浅、低分级原发加多发或复发的膀胱移行上皮癌标本及ｐ２１＾ＷＡＦ１／ＣＩＰ１蛋白的表达。结果 在１４个膀胱移行上皮癌标本均有ｐ５３基因的点突变，同一例患者所有标本的点突变分别在同一位点上；１４个膀胱移行上皮癌标本中仅有５个有ｐ２１＾ＷＡＦ１／ＣＩＰ１蛋白的表达。结论
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| 关 键 词： | 膀胱肿瘤 克隆细胞 多发 复发 |
| 修稿时间： | 1999-08-30 |
The clonal origins of recurrence or multifoci of bladder carcinoma |
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| Peter Murphy.The clonal origins of recurrence or multifoci of bladder carcinoma[J].Chinese Journal of Urology,2000,21(5):280-281. |
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| Authors: | Peter Murphy |
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| Abstract: | Objective To investigate the clonal origin of recurrence or multifoci of transitional cell carcinoma of human bladder. Methods Point mutation of p53 gene in 14 samples of superficial and low grade transitional cell carcinoma of human bladder in 6 patients were examined by PCR SSCP assay and DNA sequencing. Expression of p21 WAF1/CIP1 was measured by Western blot assay. Results Point mutation of p53 gene was found in all the 14 bladder cancers. Samples from the same patient had the identical abnormality being the common phenomenon. Of 14 samples, only 5 had positive expression of p21 WAF1/CIP1 . Conclusions Recurrence or multifoci of transitional cell carcinoma of human bladder is of single clonal origin. Regulation of p21 WAF1/CIP1 expression is mediated through either p53 dependent or p53 independent pathways. |
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| Keywords: | Bladder neoplasms Carcinoma Clone cells |
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