| 大鼠肾小管上皮细胞缺氧/复氧损伤模型的建立 |
| |
| 引用本文： | 董华,王利华. 大鼠肾小管上皮细胞缺氧/复氧损伤模型的建立[J]. 山西医科大学学报, 2008, 39(6) |
| |
| 作者姓名： | 董华 王利华 |
| |
| 作者单位： | 山西医科大学第二临床医学院肾内科,太原,030001 |
| |
| 基金项目： | 山西省自然科学基金 , 山西医科大学第二医院博士启动基金 |
| |
| 摘 要： | 目的 探讨建立大鼠肾小管上皮细胞NRK-52E缺氧/复氧损伤模型的一种新方法. 方法 本实验使用大鼠NRK-52E细胞.实验分为对照组和实验组,实验组分为缺氧2,4,6 h及缺氧后复氧1,12和24 h组.缺氧时换用缺氧液,再置入N294% O2 1% CO2 5%缺氧环境;缺氧后换用含10%胎牛血清的EMDM/F12,置入95%空气 CO2 5%有氧环境,制作缺氧/复氧模型;台盼蓝摄取法进行细胞计数及检测细胞存活率,分光光度法检测培养基中的乳酸脱氢酶(LDH)含量. 结果 大鼠肾小管上皮细胞经过缺氧/复氧处理后,与对照组相比,台盼蓝摄取率显著提高,细胞存活率显著下降,LDH含量升高,差异有统计学意义(P<0.05). 结论 采用三气孵箱法建立大鼠.肾小管上皮细胞缺氧/复氧损伤模型,较其他制模方法 操作简单、易行、可靠.
|
| 关 键 词： | 缺氧 复氧 肾小管上皮细胞 细胞模型 大鼠 肾小管上皮 细胞缺氧 复氧 损伤模型 hypoxia Establishment renal tubular epithelial cells model 操作 制模方法 孵箱 三气 统计学意义 差异 摄取率 处理 结果 含量 乳酸脱氢酶 |
Establishment of hypoxia/reoxygenation injury model in rat renal tubular epithelial cells |
| |
| DONG Hua,WANG Li-hua. Establishment of hypoxia/reoxygenation injury model in rat renal tubular epithelial cells[J]. Journal of Shanxi Medical University, 2008, 39(6) |
| |
| Authors: | DONG Hua WANG Li-hua |
| |
| Affiliation: | Dept of Nephrology;Second Clinical Medical College;Shanxi Medical University;Taiyuan 030001;China |
| |
| Abstract: | Objective To explore a new way for establishing a model of hypoxia/reoxygenation injury in rat renal tubular cell line NRK-52E. Methods Rat renal tubular cell line NRK-52E was divided into control group and experimental group.The cells in experimental group were cultured under the condition of N294%+O21% + CO25% for 2,4 and 6 h and then 95% air+CO25% for 1,12 and 24 h with 10% fetal bovine serum DMEM/F12.The cells in control group were cultured with 10% fetal bovine serum DMEM/F12 under the condition of 95%... |
| |
| Keywords: | hypoxia reoxygenation renal tubular epithelial cell cell model |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
