复发性单疱病毒性角膜炎实验模型的研究

目的：建立一种可靠、实用的复发性单疱病毒性角膜炎（HSK）的实验模型。方法：用单纯病毒I型（HSV－1）Mckrae株行NIH鼠的角膜接种，用人的抗HSV－1血清行鼠的腹腔内注射，使病毒在三叉神经节或角膜内建立起潜伏感染。用紫外线B光照射鼠的角膜，诱导HSK复发。观察诱导HSK复发的成功率、复发性HSK的临床特征、组织学特点。结果：紫外线照射诱导鼠HSK复发的成功率为72．5％，复发性HSK主要表现为基质型角膜炎，组织切片见角膜基质层内有大量的淋巴细胞和一些中性粒细胞浸润。

Study on the.experimental mouse model or recurrent herpes simplex keratitis

To establish a reliable and practical model of recurrent herpes simplex keratitis (HSK) in mouse. Methods: Latent infection in the trigemental ganglion or cornea was established by inoculating HSV-1 Mckrse strain on the cornea of NIH mouse, which was simultaneously injected intraperitonealy with 1 ml of pooled human serum containing anti-HSV-l. Ultraviolet B light (UV-B) was .used to induce the. recurrence of HSK. The recurrent rate, clinical and histological changes of HSK were investigated. Results: The recurrent rate of HSK after UV-B irradiation was 72.5%. The recurrent HSK was characterized by stromal keratitis. The infiltration in many lymphocytes and some leukocytes were found in corneal stroma. Conclusions: The animal model of recurrent HSK described here seems a relatively ideal one.