| 新等位基因HLA-B~* 9537的确认和序列分析 |
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| 引用本文： | 王东梅,单小燕,王立君,何晓玫,倪蕾,崔爽,王琳,李伟,刘娜,赵波涛,龚治尹,张志欣,宋长兴.新等位基因HLA-B~* 9537的确认和序列分析[J].中国输血杂志,2009,22(6). |
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| 作者姓名： | 王东梅 单小燕 王立君 何晓玫 倪蕾 崔爽 王琳 李伟 刘娜 赵波涛 龚治尹 张志欣 宋长兴 |
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| 作者单位： | 1. 北京市红十字血液中心,北京,100088
2. 北京市结核病胸部肿瘤研究所 |
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| 摘 要： | 目的识别并确认1个新的HLA等位基因。方法应用PCR-SSO,PCR-SSP基因分型技术对1份临床样本做HLA分型,对可能的HLA新等位基因进行分子克隆和DNA测序,分析与最同源的B*1504的差异。结果得到1份样本的序列与已知的所有HLA-B等位基因序列不一致,与B*1504的差异表现在第3外显子区域中的379G>C,409C>T,412G>A,419C>T,导致氨基酸分别由谷氨酸→天冬氨酸(E103D),苏氨酸→赖氨酸(T113K),谷氨酰胺→谷氨酸(Q114E)和丝氨酸→苯氨酸(S116F)。结论该基因为HLA-B位点的1个新等位基因,已被WHOHLA因子命名委员会正式命名为HLA-B*9537
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| 关 键 词： | HLA HLA-B*9537 新等位基因 PCR-SSO SBT序列分析 |
Identification and sequence analysis of a novel allele HLA*B*9537 |
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| WANG Dongmei,SHAN Xiaoyan,WANGLijun,et al..Identification and sequence analysis of a novel allele HLA*B*9537[J].Chinese Journal of Blood Transfusion,2009,22(6). |
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| Authors: | WANG Dongmei SHAN Xiaoyan WANGLijun |
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| Institution: | WANG Dongmei,SHAN Xiaoyan,WANGLijun,et al. Beijing Red Cross Blood Center,Beijing 100088,China |
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| Abstract: | Objective To identify a novel HLA-Ballele of a Chinese.Methods An ambiguous result was found dur-ing routine SSO and PCR-SSP typing,and molecular cloning and DNA sequence analysis were further conducted.Results The sequence of this new allele indicated that it differed from the closet matching allele B*1504 in 4 nucleotide substitu-tions,i.e. 379 G>C ,409 C >T,412 G>A,and 419 C >Tin Exon3,resulting in 4 amino acid changed from Glu to Asp(E103D),Thr to Lys(T113K),Gln to Glu(Q114E) and Ser to Phe(S116F),respe... |
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| Keywords: | HLA-B*9537 Novel allele HLA PCR-SSO SBT |
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