子前期孕妇血清及胎盘中IL-6和IL-17的表达及意义

目的通过观测子前期孕妇血清和胎盘中IL-6和IL-17水平的变化,探讨这两种细胞炎性因子与子前期发病的关系。方法采用酶联免疫吸附法（ELISA）分别测定30例子前期孕妇（子前期组）和30例正常晚期妊娠孕妇（对照组）血清中IL-6和IL-17的浓度。采用免疫组织化学法测定子前期组和对照组胎盘组织中IL-6和IL-17的表达水平。结果子前期组血清中IL-6和IL-17浓度均高于对照组,差异均有统计学意义（P〈0.01）。IL-6在子前期组胎盘中的表达明显低于对照组,差异有统计学意义（P〈0.05）。IL-17在两组胎盘中的表达差异无统计学意义（P〉0.05）。结论炎性细胞因子IL-6和IL-17与子前期的发生、发展有关。

Detection and significance of IL-6 and IL-17 in the placenta and blood in normal pregnancies and preeclampsia

Objective To study and compare the levels of interleukin-6 and interleukin-17 in serum and placenta of women with preeclampsia and normotensive pregnancy.Methods Thirty patients with preeclampsia,and 30 normotensive controls were recruited in this study.The concentrations of IL-6 and IL-17 in serum were measured by enzyme-linked immunosorbent assay（ELISA）,and their expressions in the placental specimens were detected by immunohistochemistry.Results There were no statistical differences in maternal age and gestational age between control and preeclampsia group.The neonatal weight in preeclampsia group were significantly lower than those in normal group（P0.01）.The mean concentrations of serum IL-6 and IL-17 in preeclamptic subjects were increased significantly as compared with those in healthy control pregnancy（P 0.01）.The levels of IL-6 was significantly lower（P0.05） in preeclamptic group,whereas the levels of IL-17 showed no significantly differences between two groups（P0.05）.Conclusion The inflammatory factors such as IL-6 and IL-17 may play important roles in the pathogenesis of preeclampsia.