| 金黄色葡萄球菌中毒休克综合征毒素1在大肠杆菌中的分泌型表达 |
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| 引用本文： | 祁伟,宋诗铎,李为官,兰何屏,胡文芝. 金黄色葡萄球菌中毒休克综合征毒素1在大肠杆菌中的分泌型表达[J]. 中华微生物学和免疫学杂志, 1997, 0(5) |
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| 作者姓名： | 祁伟 宋诗铎 李为官 兰何屏 胡文芝 |
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| 作者单位： | 天津医科大学第二医院传染病研究室 |
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| 基金项目： | 天津市二十一世纪青年科学基金 |
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| 摘 要： | 中毒休克综合征毒素１（Ｔｏｘｉｃｓｈｏｃｋｓｙｎｄｒｏｍｅｔｏｘｉｎ－１，ＴＳＳＴ－１）在中毒休克综合征（Ｔｏｘｉｃｓｈｏｃｋｓｙｎｄｒｏｍｅ，ＴＳＳ）的发病过程中起重要作用。本研究应用ＰＣＲ和ＤＮＡ重组技术，构建了ＴＳＳＴ－１分泌型表达载体ｐＲＴＳ２０并在大肠杆菌细胞周质表达出分子量为２２×１０３的重组ＴＳＳＴ－１，表达量约占周质总蛋白的６％～８％。Ｗｅｓｔｅｒｎ印迹杂交表明，表达产物具有特异的抗原活性。对ＴＳＳＴ－１生物学特性、结构功能关系有待进一步研究。本文对ＴＳＳＴ－１抗毒素的制备具有一定的参考意义。
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| 关 键 词： | 金黄色葡萄球菌 中毒休克综合征 基因表达 大肠杆菌 |
Secretory expression fo toxic shock syndrome toxin 1 gene of Staphylococcus aureus in E.coli |
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| Qi Wei,Song Shiduo,Li Wei,et al.. Secretory expression fo toxic shock syndrome toxin 1 gene of Staphylococcus aureus in E.coli[J]. Chinese Journal of Microbiology and Immunology, 1997, 0(5) |
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| Authors: | Qi Wei Song Shiduo Li Wei et al. |
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| Affiliation: | Qi Wei,Song Shiduo,Li Wei,et al.Department of Infectious Diseases,Second Teaching Hospital,Tianjin Medical University,Tianjin 300211 |
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| Abstract: | Toxic shock syndrome toxin 1,(TSST 1) plays a significant role in pathogenesis of toxic shock syndrome.A secretory expression plasmid pRTS20 was constructed and effectively expressed in periplasmic space in E.coli by means of PCR and DNA recombinant techniques in vitro. The recombinant TSST 1,22kD protein exhibited specific antigenicity shown by Western blotting analysis.The result of this study is of great value for further study on biological properties,structure function relationship of TSST 1 and preparation of antitoxin of TSST 1. |
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| Keywords: | Staphylococcus aureus Toxic shock syndrome Gene expression E.coli |
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