Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis. |
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| Authors: | J J Tudor L Marri P J Piggot L Daneo-Moore |
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| Affiliation: | Biology Department, Saint Joseph's University, Philadelphia, Pennsylvania 19131. |
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| Abstract: | Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were greater than 100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 +/- 60 kilobase pairs. |
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