慢性乙型肝炎病毒感染者辅助性T淋巴细胞亚群的变化

目的 了解慢性乙型肝炎病毒（HBV）感染者体内辅助性T淋巴细胞（Th细胞）亚群的变化，探讨HBV感染慢性化与Th细胞亚群变化的关系。方法 通过细胞内染色技术，采用流式细胞术检测HBV感染者外周血中Th1细胞和Th2细胞的水平。结果 慢性HBV感染者中，乙型肝炎病毒e抗原（HBeAg）阳性感染组和HBV-DNA复制组Th1数量明显低于健康对照组（9．46±2．06，9．52±2．41与11．18±3．12相比，P〈0．05）；HBeAg阳性组、丙氨酸氨基转移酶（ALT）升高组、HBV-DNA复制组Th2细胞数量明显高于健康对照组（3．05±1．12，3．12±1．12，2．97±1．12与2．38±0．96相比，P〈0．05）；HBeAg阳性感染组、ALT升高组和HBV-DNA复制组Th1／Th2比值都明显低于健康对照组差异有统计学意义（P〈0．05）。结论 慢性HBV感染者Th1／Th2比例失衡，表现为Th1／Th2比值下降，Th2细胞数量优势，Th1／Th2比例失衡可能与肝脏损伤程度和病毒复制水平有关，Th1／Th2比值可以作为监测HBV慢性感染者免疫状态的重要指标。

Variance of helper lymphocyte T cell subsets in patients with chronic hepatitis B virus infection

Objective To investigate the variance of helper lymphocyte T cell subsets in patients with chronic hepatitis B virus （HBV） infection, and to explore the relationship of chronicity of HBV infection and variance of helper lymphocyte T cell subsets. Methods Intracellular staining technique and flow cytometry were employed to measure peripheral blood levels of Th1 and Th2 cells. Results The percentages of Th1 cells in HBeAg（＋） group and HBV-DNA replicating group were significantly lower than that of control group （9.42±2.03,9.52 ±2. 41 vs 11.18±3.12,P〈0.05）. The percentages of Th2 cells in HBeAg （＋） group, ALT increasing group, HBV-DNA replicating group were significantly higher than that of control group （3.05±1.12, 3. 12±1. 12, 2.97±1. 12 vs 2.38±0.96,P〈0.05）. All the Th1/Th2 ratios in HBeAg（＋） group, ALT increasing group and HBV-DNA replicating group were significantly lower than that of control group （P〈0.05）. Conelusion There exists Th1 Th2 ratio imbalance in chronic HBV infection, displaying Th1/Th2 ratio decreases and Th2 cell prevails. The imbalance of Th1/Th2 ratio may be associated with degree of hepatic injury and viral replication. Th1/Th2 ratio can be used as an important index to monitor the immune state in patients with HBV infection.