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| Survivin反义核酸治疗人大肠癌裸鼠皮下种植瘤的实验研究 | |
| 引用本文： | 宫向前,乔广东,李玉华,邢建华,寿楠海,丁印鲁.Survivin反义核酸治疗人大肠癌裸鼠皮下种植瘤的实验研究[J].中国现代普通外科进展,2006,9(4):219-221. |
| 作者姓名： | 宫向前 乔广东 李玉华 邢建华 寿楠海 丁印鲁 |
| 作者单位： | 1. 山东省烟台毓磺顶医院,肿瘤外科,山东,烟台,264000 2. 山东大学齐鲁医院,普通外科,山东,济南,250012 3. 山东大学第二医院,普通外科,山东,济南,250033 |
| 基金项目： | 山东省医药卫生科技发展计划青年基金资助项目(43号) |
| 摘 要： | 目的探讨Survivin反义寡核苷酸对裸鼠人大肠癌皮下种植瘤体生长的抑制作用及其机制。方法接种人大肠癌细胞制作裸鼠皮下大肠癌种植瘤模型,分别用Survivin反义寡核苷酸、正义核苷酸和生理盐水对皮下瘤体内直接进行注射治疗,检测肿瘤的生长状况、瘤体体积及瘤重变化,计算抑制率。病理切片免疫组化检测瘤体E-CD表达。结果经Survivin硫代反义寡核苷酸治疗组裸鼠皮下移植瘤生长受到抑制,肿瘤重量明显小于对照组,肿瘤体积明显小于对照组和正义组(P<0.05),裸鼠皮下移植瘤E-CD表达增强。结论Survivin反义核酸可以抑制大肠癌裸鼠移植瘤的生长,Survivin反义核酸抑制大肠癌裸鼠移植瘤生长的机理可能与E-CD基因表达变化有关。 |
| 关 键 词： | 寡核苷酸类 反义·基因 Survivin·结直肠肿瘤·小鼠 裸 |
| 文章编号： | 1009-9905(2006)04-0219-03 |
| 收稿时间： | 11 9 2005 12:00AM |
| 修稿时间： | 2005年11月9日 |
The effect of survivin antisense oligodeoxynucleotides on the growth of colorectal carcinoma transplanted in nude mice |
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| GONG Xiang-qian,QIAO Guang-dong,LI Yu-hua,XING Jian-hua,SHOU Nan-hai,DING Yin-lu.The effect of survivin antisense oligodeoxynucleotides on the growth of colorectal carcinoma transplanted in nude mice[J].Chinese Journal of Current Advances in General Surgery,2006,9(4):219-221. | |
| Authors: | GONG Xiang-qian QIAO Guang-dong LI Yu-hua XING Jian-hua SHOU Nan-hai DING Yin-lu |
| Institution: | 1 Department of Surgical Oncology, Yuhuangding Hospital of Yantai city ,Yantai 264000 ,China; 2 Department of General Surgery, Qilu Hospital of Shandong University ,Jinan 250012, China; 3 Department of General Surgery, the Second Hospital of Shandong University ,Jinan 250033, China |
| Abstract: | Objective:To study the inhibitory effect of survivin antisense oligodeoxynucleotides on growth of human colorectal carcinoma in nude mice.Methods:Human colorectal carcinoma transplanted subcutaneously in nude mice model was estabished.A total of 24 mice were di-vided into three groups,namely control group,survivin-SODN group and survivin-ASODN group.Different treatments were given respectively,continuous infusions over 16 days.The weight and volume of the subcutaneous tumors was measured,and the morphology of tumor was observed.The expression of E-cadherin was examined immunohistochemically in the human colorectal cancer transplanted subcutaneously in nude mice.Results:The growth inhibitory rate was 57%in ASODN group,while only 6.67% was in SODN group.The weight and volume of the subcutaneous tumors in survivin-ASODN group is smaller than in control group.And the expression of E-cadherin is stonger in the ASDON group than in control group.Conclusion:Survivin antisense oligodeoxynucleotides are powerful of inhibiting the growth of human colorectal carcinoma in nude mice.The change of E-cadherin,another tumor-related gene,may play an important role in this progress. |
| Keywords: | Ollsodeoxynucleotides antisense Genes survivin Coloreotal neoplasms Mice node |
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