酸浆提取物改善老龄大鼠抗氧化功能的研究

目的研究酸浆提取物对D-半乳糖致老大鼠抗氧化能力的影响。方法将6周龄Wistar雄性大鼠75只,随机分为青年对照组、模型组和低(10 mg/kg)、中(15 mg/kg)、高(20 mg/kg)剂量酸浆提取物处理组,另以15只16月龄同品系大鼠设为正常老年对照组。其中对模型组和提取物处理组用D-半乳糖进行皮下连续注射5个月。5个月后分别对各组大鼠血清丙二醛(MDA)含量、肝脏匀浆MDA含量、血液超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)酶活性进行测定并进行组间比较。结果与青年对照组相比,模型组血清MDA含量和肝脏匀浆MDA含量均比对照组有显著升高(P<0.01),血液SOD、GSH-Px酶活性比对照组有显著降低(P<0.01),与老龄对照组变化趋势呈现一致;而各提取物处理组血清MDA含量和肝脏匀浆MDA含量均比模型组有显著降低(P<0.01),血液SOD、GSH-Px酶活性比模型组有显著升高(P<0.01)。结论摄入适量酸浆提取物可以有效地增强大鼠机体抗氧化能力,从而延缓D-半乳糖诱发的大鼠衰老。

Improving effect of ground cherry's extraction on antioxidant ability in aging rats

Objective To study the influence of ground cherry's extraction on antioxidant ability in aging rats induced by D-galactose.Methods All 75 Wister male rats(6 weeks old) were randomly divided into five groups,including the young control group(group 1),model group(group 2),low-dose extraction group(10 mg/kg,group 3),middle-dose extraction group(15 mg/kg,group 4) and high-dose extraction group(20 mg/kg,group 5),besides other 15 Wister male rats(16 months old) were included in the old control group(group 6).The rats in group 2,3,4 and 5 were given injection of D-galactose for 5 months.After 5 months the contents of serum malonaldhydic acid(MDA) and liver MDA,activities of superoxide dismutase(SOD) and glutathion peroxidase(GSH-Px) in all groups were detected and compared.Results Compared with the young control group,the content of serum MDA,liver MDA were significantly increased(P<0.01) and the activities of SOD and GSH-Px were significantly decreased(P<0.01) in group 2,but those changes were as the same as those of group 6.The contents of serum MDA and liver MDA in group 3,4 and 5 were significantly decreased compared with those in group 2(P<0.01),while the activities of SOD and GSH-Px were significantly increased(P<0.01).Conclusion The extraction of ground cherry can increase the antioxidantive ability of rats in order to delay the consenescence induced by D-galactose.