| RP-HPLC法测定蒲公英叶及根中咖啡酸的含量 |
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| 引用本文： | 程铁峰,朱新科,许启泰. RP-HPLC法测定蒲公英叶及根中咖啡酸的含量[J]. 河南大学学报(医学版), 2006, 25(4): 59-61 |
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| 作者姓名： | 程铁峰 朱新科 许启泰 |
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| 作者单位： | 1. 河南大学,中药研究所,河南,开封,475001
2. 开封市河大制药厂,河南,开封,475001 |
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| 摘 要： | 目的:采用RP-HPLC对蒲公英叶及根中咖啡酸的含量进行测定。方法:选用Hyper ODS C18色谱柱(250mm×4.6 mm),甲醇-磷酸盐缓冲液(23∶77)为流动相,紫外检测波长为323 nm,柱温:40℃条件下对咖啡酸进行含量测定。结果:咖啡酸的含量在0.145μg~0.726μg范围内,进样量与峰面积积分值呈良好的线性关系,r=0.9996,平均回收率98.3%,RSD=1.80%。结论:此方法能准确、快速地进行定量检测,可作为蒲公英叶及根中咖啡酸的质量控制方法。
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| 关 键 词： | 咖啡酸 RP-HPLC 蒲公英 |
| 文章编号： | 1672-7606(2006)04-0059-03 |
| 收稿时间： | 2006-11-08 |
| 修稿时间： | 2006-11-08 |
Determination of caffeic acid in leaves and roots of Herba taraxacum mongolicum by RP-HPLC |
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| CHENG Tie-feng,ZHU Xin-ke,XU Qi-tai. Determination of caffeic acid in leaves and roots of Herba taraxacum mongolicum by RP-HPLC[J]. Journal of Henan University, 2006, 25(4): 59-61 |
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| Authors: | CHENG Tie-feng ZHU Xin-ke XU Qi-tai |
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| Abstract: | Objective: To develop a RP-HPLC method for the caffeic acid in leaves and roots of Herba taraxacum mongolicum,which determined the quantity of caffeic acid.Methods: The chromatographic conditions included Hyper ODS C18 column(250 mm×4.6 mm) and the mobile phase consisted of methanol-phosphate buffer solution(23∶77).The detection wavelength was at 323 nm.Results: Caffeic acid showed a good linear relation ship at the rang of 0.145 μg～0.726 μg,r = 0.9996.The average recovery rate was 98.3% and RSD was 1.80%.Conclusion: The methods are accurate and quick,and can be used for the quality control of caffeic acid in leaves and roots of Herba taraxacum mongolicum. |
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| Keywords: | Caffeic acid RP-HPLC Herba taraxacum mongolicum |
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