Survivin蛋白在乳腺癌发生、发展不同阶段的表达及意义

背景与目的:Survivin蛋白是新近发现的凋亡抑制因子,主要通过抑制Caspase-3、Caspase-7而阻断细胞凋亡过程,目前研究表明Survivin蛋白在很多恶性肿瘤组织中高表达。本研究通过观察Survivin在正常乳腺组织、乳腺囊性增生组织、乳腺不典型增生组织及乳腺癌组织中的蛋白表达,探讨Survivin在乳腺癌发生、发展中的作用。方法:采用免疫组织化学SP法检测正常乳腺组织(96例)、乳腺囊性增生组织(56例)、乳腺不典型增生组织(12例)及乳腺癌组织(119例)中Survivin蛋白的表达,并分析其和乳腺癌临床病理特征的关系。结果:Survivin蛋白在正常乳腺组织、乳腺囊性增生组织、乳腺不典型增生组织及乳腺癌组织中的阳性率分别为4.2%(4/96)、5.4%(3/56)、42.7%(5/12)、72.2%(86/119),后二者的阳性率明显高于前二者(P<0.005);浸润性非特殊类型乳腺癌Survivin蛋白阳性率(82.0%,73/89)明显高于浸润性特殊型乳腺癌(45.4%,10/22)和早期浸润性乳腺癌(37.5%,3/8)(P<0.05);Survivin蛋白表达在有淋巴结转移的乳腺癌中有升高的趋势,但差异无统计学意义(P>0.05)。结论:Survivin蛋白在乳腺癌发生、发展不同阶段的表达呈进行性上升的趋势,过度表达提示预后不良。

Expression of survivin in different stages of carcinogenesis and progression of breast cancer

BACKGROUND &OBJECTIVE: Survivin is one of the newly identified apo ptosis inhibitor; it can block the cell apoptosis by inhibiting the function of the enzyme caspase-3 and caspase-7. Present studies indicate that survivin is overexpressed in malignant tumor. This current study was designed to investigate the effects of survivin in tumorigenesis and progression of breast carcinoma th rough observing the expression of survivin in the tissue of normal mammary, cyst ic hyperplasia mammary, atypical hyperplasia mammary and breast carcinoma. METHO DS: The expression of survivin in the tissue of normal mammary (96 cases), cysti c hyperplasia mammary (56 cases), atypical hyperplasia mammary (12 cases), and b reast carcinoma (119 cases) were evaluated by SP immunohistochemistry. The relat ionship between survivin expression and the pathologically biological features o f breast cancer was assessed. RESULTS: The positive rates of survivin were 4.2% (4/96),5.4%(3/56),42.7%(5/12), and 72.3%(86/119)in the tissue of normal mam mary, cystic hyperplasia mammary, atypical hyperplasia mammary, and breast carci noma, respectively. The positive rates in the last two groups were higher than t hose in the former two groups (P< 0.005). Survivin was more expressed in the inf iltrative nonspecial breast carcinoma (82.0%, 73/89) than in the special and ea rly stage infiltrative breast carcinoma (37.5%,3/8)(P< 0.05). Expression of sur vivin was correlated with lymph node metastasis although the difference is not s ignificant (P >0.05). CONCLUSION: Overexpression of survivin is common in tumori genesis and progression of breast carcinoma. Altered expression of survivin may contribute to tumorigenesis and progression of breast carcinoma by inhibiting ce ll apoptosis, its overexpression indicates worse prognosis.