外周血T细胞亚群及细胞因子对外源性哮喘IgE生成的作用

对３０例哮喘患者及３０例健康成年人的外周血，采用单克隆抗体（ＭｃＡｂ）间接免疫荧光法测定Ｔ细胞亚群；ＥＬＩＳＡ双抗体夹心法测定ＩｇＥ、ＩＬ－４；ＦＩ２细胞株，生物学方法测定ＩＬ－２；ＩＬ－６依赖细胞株７ＴＤ１，掺入法测定ＩＬ－６；用抗人ＣＤ２３的ＭｃＡｂ测定ＣＤ２３。为研究Ｔ细胞、细胞因子对哮喘ＩｇＥ生成调节机理及细胞因子在哮喘发病过程中的作用。结果显示：发作期ＩｇＥ、ＩＬ－２、ＩＬ－４、ＣＤ２３、ＣＤ８~＋、ＣＤ４／ＣＤ８~＋比值较对照组及缓解期有显著性差异（Ｐ＜０．０１）。缓解期ＩｇＥ与对照组之间无显著性差异（Ｐ＞０．０５）；ＣＤ８＋、ＣＤ４／ＣＤ８比值，ＣＤ２３与对照组之间有显著性差异（Ｐ＜０．０１）。ＣＤ２３、ＣＤ４、ＩＬ－６三组间无显著性差异（Ｐ＞０．０５）。结果表明，ＩｇＥ合成增加是哮喘发作的关键，Ｔ细胞对日细胞合成ＩｇＥ的调节是通过细胞因子实现的，细胞因子又参与气道炎症过程。

Mechanism of T cell subsets and cytokines in the regulation of IgE production in exogenous asthma

The peripheral blood of 30 cases of asthma and 30 control adults were measured for T cell subsets with indirect Immunofluorescence of monoclonal antibodies , for IgE , IL-4 with ELISA , for IL-2 with FI2-cell line-biological method , for IL-6 with IL -6-dependent cell line 7TD1. mtake method and for CD23 with anti human CD23 McAb. The mechanism of T cells and cytokines in the regulation of IgE production in asthma and the effect of cytokines on the pathogenesis of asthma were also studied. The results showed that the levels of IgE ,IL-4 ,IL-2 ,CD23 ,CD8 ,as well as the ratio of CD4/CD8 in cases of their acute stage were significantly different from those in their remission stage and normal controls (P<0.01) .In their remission stage, there was no significant IgE difference between cases and control(P>0.05). And there were significant differences of CD8 ,CD4/CD8 ratio between cases and normal controls (P<0.01). There was no significant difference of CD3 ,CD4 , IL-6 among three groups (P>0.05). It indicated that the increased production of IgE antibody Was the key factor in the pathogenesis of exogenous asthma and the cytokines played roles. in the process of inflammatory reactions in the airway.