白藜芦醇对大鼠肠缺血再灌注损伤的保护作用

目的 探讨白藜芦醇(resveratro1,RES)对实验性大鼠肠缺血再灌注肠黏膜损伤的保护作用.方法 24只成年雄性SD大鼠随机分为假手术组(SO)、缺血再灌注损伤组(I/R)、RES治疗组.SO组仅分离肠系膜上动脉(SMA)根部而不夹闭.肠缺血再灌注损伤组(I/R)和RES治疗组均用无损伤血管夹夹闭SMA根部后分别立即经阴茎背静脉注射生理盐水、RES(20 mg/kg),45 min之后放松血管夹形成再灌注.各组大鼠均于制模后6 h采集标本.检测血清和回肠组织中的超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,TUNEL法检测肠黏膜上皮细胞凋亡率,并观察肠黏膜病理变化.结果 小肠缺血再灌注后,血清及小肠组织中反映氧化损伤程度的MDA明显升高,SOD则明显减少,小肠黏膜上皮细胞凋亡,应用RES后能显著改善上述改变.结论 白藜芦醇对肠缺血再灌注损伤具有保护作用,其机制可能与通过抗氧化作用及抑制肠黏膜上皮细胞凋亡有关.

Abstract：

Objective To study the protective effects of resveratrol (RES) on intestinal ischemia-reperfusion injury in rats.Methods Twenty-four SD rats were randomly divided into the sham operation group,intestinal ischemia-reperfusion injury (I/R) group and resveratrol treated (RES) group.The intestinal ischemia injury was induced by superior mesenteric artery occlusion for 45 minutes,and then the blood supply to the intestine was restored to cause reperfusion injury.After 6 hours' reperfusion,the rats were sacrificed and intestine was collected.Of the RES group,the rats were subjected to I/R injury,and treated with 20 mg/kg resveratrol by intravenous injection immediately after the mesenteric artery was clamped.Superoxide Dismutase (SOD) and Malonaldehyde (MDA) levels in serum and intestine were measured.Apoptotic intestinal epithelial cells were detected by TUNEL methods.The histological injury of the intestine was also examined.Results Compared with the sham operated rats,MDA levels in the serum and intestine as well as the apoptotic epithelial cells were significantly increased in the rats subjected to I/R (MDA in serum and intestine 4.63±0.53 vs 1.32±0.40;8.60± 0.98 vs 4.13±0.86,P＜0.01;apoptotic index 66.63 ± 1.71 vs 46.72 ± 1.50,P＜0.01 ).However,the SOD levels in the serum and intestine were decreased (49.21±4.38 vs 86.65±6.14;351.03 ± 21.46 vs 468.93 ± 16.21,P＜0.01).In the rats subjected to I/R injury but received resveratrol treatment,the epithelial cells apoptosis and MDA levels in serum and intestine were decreased,and SOD levels in serum and intestine increased (P＜0.05).Conclusions Resveratrol protects intestine from ischemia-reperfusion injury in rats.

Proctective effects of resveratrol on intestinal ischemia-reperfusion injury in rats

Objective To study the protective effects of resveratrol (RES) on intestinal ischemia-reperfusion injury in rats.Methods Twenty-four SD rats were randomly divided into the sham operation group,intestinal ischemia-reperfusion injury (I/R) group and resveratrol treated (RES) group.The intestinal ischemia injury was induced by superior mesenteric artery occlusion for 45 minutes,and then the blood supply to the intestine was restored to cause reperfusion injury.After 6 hours' reperfusion,the rats were sacrificed and intestine was collected.Of the RES group,the rats were subjected to I/R injury,and treated with 20 mg/kg resveratrol by intravenous injection immediately after the mesenteric artery was clamped.Superoxide Dismutase (SOD) and Malonaldehyde (MDA) levels in serum and intestine were measured.Apoptotic intestinal epithelial cells were detected by TUNEL methods.The histological injury of the intestine was also examined.Results Compared with the sham operated rats,MDA levels in the serum and intestine as well as the apoptotic epithelial cells were significantly increased in the rats subjected to I/R (MDA in serum and intestine 4.63±0.53 vs 1.32±0.40;8.60± 0.98 vs 4.13±0.86,P＜0.01;apoptotic index 66.63 ± 1.71 vs 46.72 ± 1.50,P＜0.01 ).However,the SOD levels in the serum and intestine were decreased (49.21±4.38 vs 86.65±6.14;351.03 ± 21.46 vs 468.93 ± 16.21,P＜0.01).In the rats subjected to I/R injury but received resveratrol treatment,the epithelial cells apoptosis and MDA levels in serum and intestine were decreased,and SOD levels in serum and intestine increased (P＜0.05).Conclusions Resveratrol protects intestine from ischemia-reperfusion injury in rats.