针刺对局灶性脑缺血大鼠脑细胞凋亡及Bcl-2蛋白表达的影响

目的 :研究针刺在大鼠局灶性脑缺血中的脑保护作用及分子机制。方法 :采用线栓法建立大鼠大脑中动脉缺血 (MCAO) 2 4hr模型 ,40只雄性大鼠随机分为脑缺血组和脑缺血 +针刺组 ,每组 2 0只 ,采用免疫组织化学方法观察两组脑细胞凋亡数与Bcl 2蛋白表达。结果 :脑缺血 +针刺组细胞调亡数 ( 3 .1 6± 1 .1 2个 /视野 )低于脑缺血组 ( 7.2 3± 1 .50个 /视野 ) ,P <0 .0 5。Bcl 2蛋白染色阳性细胞数 ( 89± 1 4个 /mm2 )高于脑缺血组 ( 42± 2 1个 /mm2 )P <0 .0 5。结论 :针刺可明显减轻局灶性脑缺血损伤 ,其提高Bcl 2蛋白的表达 ,进而抑制细胞凋亡 ,是其脑保护作用的分子机制之一。

Effect of Acupuncture on Apoptosis and Expression of Bcl-2 Protein in Rats with Local Cerebral Ischemia

Objective:To observe the effect of acupuncture on ischemic cerebral neurons and to investigate its molecular mechanisms in reducing cerebral ischemic injury. Methods: Forty SD rats were randomly and evenly assigned to model group and acupuncture group. Cerebral ischemia model was established by middle cerebral artery occlusion. Electroacupuncture (EA, 2 Hz, 1 mA, duration of 60 min) was applied to “Shuigou”(GV 26), “Neiguan”(PC 6) and “Baihui”(GV 20). Sections of the cerebral tissue were stained by using immunohistochemical (ABC) method. The number of apoptosis and Bcl 2 expression were observed under microscope. Results: The mean numbers of apoptotic cells of the local ischemic cerebral region in model group and acupuncture group were 7.23±1.50 and 3.16±1.12 respectively, showing that the apoptotic cells of acupuncture group were significantly fewer than those of model group. The mean numbers of Bcl 2 immunoreactive (IR) positive neurons of model and acupuncture groups were 42±21 and 89±14 separately, with the number of Bcl 2 IR positive neurons of acupuncture group being markedly more than that of model group ( P <0.05). Conclusion: Acupuncture could apparently suppress cerebral ischemia induced apoptosis and up regulate Bcl 2 protein expression,that may contribute to the protective effect of acupuncture on ischemic cerebralneurons.