人胃癌中Survivin基因的克隆及其剪切体的发现

目的：克隆人类抗凋亡基因survivin(SVV)。方法：提取胃癌细胞系SGC－7901总RNA，RT－PCR扩增人SVV全长cDNA，测序鉴定、原核表达。结果：得到两个SVV基因cDNA克隆，即SVV－S4A与SVV－S1B，前者与已知SVV基因cDNA序列相同，后者第3个外显子丢失。原核表达所获融合蛋白经SDS－PAGE电泳与已知SVV基因的蛋白质表达产物大小相近。结论：自人胃癌细胞中克隆出SVV基因的全长cDNA，并获得一个新的剪切体，实现了SVV基因在大肠杆菌BL21（DE3）中的高效表达。

Cloning and finding of its new splicer of survivin gene from human gastric cancer

Objective:The purpose of this paper is to clone survivin(SVV) gene from human gastric cancer. Methods: The SVV cDNA was obtained by using RT PCR method with total RNA extracted from the human gastric cancer cell line, SGC7901. After proved to be correct by sequencing , recombinant expression plasmid p RSET SVV was transformed into E.coli BL21(DE3). The fusion protein was produced by IPTG induction. Results : Obtained two SVV cDNA clones , SVV S 4 A and SVV S 1 B. The sequence of the previous is identical to that of the well known SVV, but , the third exon was missed in the latter.A new anticipated M r 19 500 protein band appeared on SDS PAGE gel induced by IPTG. Conclusion: human SVV cDNA was cloned and a new splicer is found from human gastric cancer. SVV is highly expressedsioned in E. coli.