| 激活态雪旺细胞在细胞培养下的生长规律 |
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| 引用本文： | 劳杰,顾玉东. 激活态雪旺细胞在细胞培养下的生长规律[J]. 中华手外科杂志, 2002, 18(3): 174-176 |
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| 作者姓名： | 劳杰 顾玉东 |
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| 作者单位： | 200040,上海,复旦大学华山医院手外科 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 170 96 5)，国家 973创伤基础研究基金资助项目 (G19990 54 2 0 2 )，国家教委优秀青年教师基金资助项目，上海市曙光计划资助项目，国家教育部留学归国人才基金资助项目 |
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| 摘 要： | 目的 了解激活态雪旺细胞在细胞培养下的生长规律。方法 应用改良的雪旺细胞培养法体外培养激活态雪旺细胞 ,通过激活态雪旺细胞在各生长点 (3d ,5d,7d ,9d,1 1d ,1 3d)的计数 ,绘制激活态雪旺细胞的生长曲线。作Lowry蛋白测定 ,H3 胸腺嘧啶核甙测定 ,了解激活态雪旺细胞在细胞培养下的生长情况。结果 每个时间段检测激活态雪旺细胞的生长繁殖能力 ,Lowry蛋白含量 ,H3 胸腺嘧啶核甙的含量 ,均数倍于正常态的雪旺细胞 ;两者差异均有非常显著性意义 (t =2 2 3、67 1、72 4,P <0 .0 0 1 )。结论 激活态雪旺细胞在细胞培养下具有旺盛的生长繁殖能力
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| 关 键 词： | 生长规律 激活态许旺氏细胞 细胞培养 周围神经再生 |
| 修稿时间： | 2002-06-11 |
Growing rule of the activated Schwann cells in culture |
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| LAO Jie,GU Yudong. Growing rule of the activated Schwann cells in culture[J]. Chinses Journal of Hand Surgery, 2002, 18(3): 174-176 |
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| Authors: | LAO Jie GU Yudong |
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| Affiliation: | LAO Jie,GU Yudong. Department of Hand Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China |
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| Abstract: | Objective To explore the growing rule of the activated Schwann cells from adult SD rat in culture.Methods The activated Schwann cells were cultured in vitro using modified culture method. The numbers of activated Schwann cells were counted at various growing point(3 d, 5 d, 7 d,9 d,11 d,13 d),then the growing curve of activated Schwann cells was drawn. The Lowry protein and thymine nucleoside were checked to examine the growth of activated Schwann cells in culture. Results The proliferation of activated Schwann cells ,the Lowry protein and thymine nucleoside are several folds more than the normal Schwann cells at each growing point. There was statistically significant difference between them. Conclusion The activated Schwann cells can proliferate highly in cell culture. |
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| Keywords: | Schwann cells Cell cultured Rats Sprague Dawley Activated Schwann cells |
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