| 甲型肝炎早期实验诊断的研究 |
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| 引用本文： | 尚守礼,丛树梅,李莉,陈虹,徐皖苏,王耀宗. 甲型肝炎早期实验诊断的研究[J]. 山东大学学报(医学版), 1989, 0(4) |
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| 作者姓名： | 尚守礼 丛树梅 李莉 陈虹 徐皖苏 王耀宗 |
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| 作者单位： | 山东医科大学传染病学教研室，济南市传染病医院 |
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| 摘 要： | 以人胚肺二倍体细胞分离出的甲型肝炎病毒作抗原,以马抗人IgM经纯化后的抗体为捕捉抗体,以纯化甲型肝炎恢复血清为特异性抗体,过碘酸钠法标记酶,以OPD为底物制成抗-HAV IgM诊断试剂盒,建立了ELISA捕捉法检测抗-HAV IgM,结果:甲型肝炎暴发点84例均为阳性,流行性出血热及健康成人各30例均为阴性。322例急性黄疸型肝炎住院患者中192例阳性(占59.6%)。12例第一病日患者11例阳性(占91.6%),证明该方法对甲型肝炎早期诊断有重要价值。
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| 关 键 词： | 甲型肝炎 甲型肝炎病毒 甲型肝炎病毒抗原 血清诊断 |
INVESTIGATION OF EARLY LABORATORY DIAGNOSIS FOR HEPATITIS A |
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| Shang Shouli,et al Dept.of Infectious Disease,Shandong Medical University. INVESTIGATION OF EARLY LABORATORY DIAGNOSIS FOR HEPATITIS A[J]. Journal of Shandong University:Health Sciences, 1989, 0(4) |
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| Authors: | Shang Shouli et al Dept.of Infectious Disease Shandong Medical University |
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| Abstract: | An anti-HAV IgM test kit was prepared by using HAV cul-tured indiploid cells from human fetal lung as antigen,commercial purifiedhorse to human IgM as capture antibody,purified serum from patients withhepatitis A(HA)in convalescence as specific antibody enzyme-labeled withsodium periodicate and PPD as substrate.A method for determining the anti-HAV IgM with ELISA IgM antibody eapture assay was established.The testshowed that the 84 patients with HA in epidemic area were positive,30cases of health adults and with epidemic hemorrhagic fever were negativeand 192 out of 322 in patients with acute jaundice hepatitis were positive(59.6%).11 out of 12 patients at first illnessday were positive(91.6%). |
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| Keywords: | Hepatitis A Hepatitis A virus Hepatitis A virus antigen Serodiagnosis |
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