大豆分离蛋白对果蝇寿命的影响

背景大豆蛋白具有降血脂、抑制肿瘤以及雌激素样作用等各种保健功效,但有关大豆分离蛋白对机体寿命影响的研究少见报道.目的观察大豆分离蛋白对果蝇寿命的影响,以探讨其抗衰老与抗氧化作用机制.设计以果蝇为研究对象的对照实验研究.单位一所大学公共卫生学院营养与食品卫生教研室.材料实验于2002-03/06在新疆医科大学公共卫生学院营养与食品卫生教研室果蝇室完成.美国野生型黑腹果蝇400只,雌、雄各半,由新疆医科大学公共卫生学院营养与食品卫生教研室提供.方法将400只果蝇分为1个对照组(普通培养基)和3个剂量组(分别为基础培养基含0.2%,1.0%,5.0%大豆分离蛋白).从实验第2天起,每天观察记录果蝇生存数和死亡数,直到全部果蝇死亡为止.计算果蝇平均寿命、半数死亡期和最高寿命. 主要观察指标果蝇平均寿命、半数死亡期和最高寿命.结果与对照组相比,大豆分离蛋白明显延长了雌雄果蝇寿命,并呈剂量反应关系,以高剂量组作用明显,其中雌雄果蝇平均寿命分别延长24.5%和20.7%;半数死亡时间分别延长27.1%和22.0%;最高寿命分别延长13.9%和10.6%.结论大豆分离蛋白能延长果蝇寿命,可能具有一定的延缓衰老作用.

Effect of isolated soybean protein on the lifespan of drosophila melanogasters

BACKGROUND: Many data demonstrate that the components of soybean can lower blood lipid,suppress the growth of cancer cells and exert weak estrogenic activities. However,little is known about the effect of isolated soybean protein on the lifespan of the organism.OBJECTIVE: To observe the effect of isolated soybean protein on the lifespan of drosophila melanogasters and investigate the mechanism of effective anti-aging and anti-oxidation action.DESIGN: A controlled trial based on drosophila melanogasters.SETTING: Department of nutrition and food hygiene in a university.MATERIALS: The experiment was conducted in the Department of Nutrition and Food Hygiene,Public Health College of Xinjiang Medical University from March to June 2002. A total of 400 drosophila melanogasters of American wild type with half for each gender were provided by the Department of Nutrition and Food Hygiene, Public Health College of Xinjiang Medical University.METHODS: The 400 drosophila melanogasters were divided into control group(normal culture) and three-dosage experiment groups(normal culture contained isolated soybean protein 0.2%,1.0% and 5.0%,respectively).From the second day on, the number of living and dead drosophila melanogasters was observed and counted until all died. Meanwhile, mean lifespan,half death time and maximal lifespan were calculated.MAIN OUTCOME MEASURFS: Mean lifespan, half death time and maximal lifespan of the drosophila melanogasters.RESULTS: Compared with that of control group, the lifespan of male and female drosophila melanogasters in experiment groups was prolonged by isolated soybean protein and responded in a dose-dependent manner,especially in high-dosage group. The mean lifespan, half death time and maximal lifespan of both female and male drosophila melanogasters were prolonged by 24.5% and20.7%,27.1% and22.0%,and 13.9% and 10.6%,respectively.CONCLUSION: Isolated soybean protein may have anti-aging and lifespan-prolonging effects on drosophila melanogasters.