Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene |
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Authors: | Yugueros J Temprano A Sánchez M Luengo J M Naharro G |
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Affiliation: | Departamento de Sanidad Animal, Microbiología e Inmunología, Universidad de León, 24071 León, Spain. |
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Abstract: | Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity. |
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