诺帝对肺腺癌细胞系A549细胞凋亡的诱导作用

目的探讨化学合成物诺帝对人肺腺癌细胞系A549细胞凋亡和凋亡调控基因bcl-2蛋白表达的影响。方法采用自行合成的化合物诺帝（终浓度为100umol／L）处理A549细胞，于处理后12、24、48和72h用细胞培养、流式细胞仪、免疫组化及图像分析等方法检测诺帝对A549细胞凋亡和bcl-2蛋白的表达。结果一定浓度的诺帝处理A549细胞12～48h，均可诱导A549细胞发生凋亡，且随着作用时间的延长，凋亡细胞数增加越明显；诺帝处理A549细胞后，出现细胞bcl-2蛋白表达水平显著降低。结论诺帝诱导了A549细胞的凋亡，其作用可能与其下调了bcl-2蛋白的表达有关。

Apoptosis of human pulmonary adenocarcinoma cell line A549 induced by Nordy

Objective To investigate the effect of synthesized Nordy on apoptosis of human pulmonary adenocarcinoma cell line A549 and the expression of apoptosis-regulated protein bcl-2. Methods Nordy （ 100 umol/L） was used to treat A549 cells. After treatment by Nordy for 12, 24, 48 and 72 hours, flow cytometry, immunohistochemistry and image analysis were applied for observation and measurement of apoptosis and bcl-2 protein expression. Results Apoptosis of A549 cells was induced by Nordy at a concentration of 100umol/L in a time-dependent manner. The expression of bcl-2 protein in A549 cells was decreased after the treatment of 100/zmol/L. Conclusion Nordy can induce apoptosis of human pulmonary adenocarcinoma cell line A549, which may be related to the down-regulation of bcl-2 protein expression.