血管内皮生长因子基因转染促进成骨细胞增殖及其功能的实验研究

目的探讨外源性血管内皮生长因子（VEGF）基因表达在成骨细胞增殖、分化中的作用。方法利用阳离子脂质体Lipofectamine将pBLAST49-mVEGF基因导人体外培养的成骨细胞，观察外源性VEGF在成骨细胞内的表达，及其对成骨细胞生长曲线、细胞周期和成骨细胞分泌骨钙素和Ⅰ型胶原的影响。结果同步培养后第1、2d，pBLAST49-mVEGF基因转染组细胞数大于对照组，二者间差异无统计学意义（P〉0．05）；从第3d开始，二者间差异具有统计学意义（P〈0．05）。pBLAST49-mVEGF基因转染组第1～5代细胞（G2／M＋S）％明显高于对照组，差异具有统计学意义（P〈O．05）。pBLAST49-mVEGF基因转染组第1～5代细胞合成分泌骨钙素浓度和Ⅰ型胶原的表达均明显高于对照组，差异具有统计学意义（P〈0．05）。结论pBLAST49-mVEGF基因转染能促进成骨细胞的增殖和成骨活性。

Experimental Study on the Proliferation and Function of Osteoblast Cell Induced by pBLAST49-mVEGF Gene Transfection

OBJECTIVE: To assess the effects of extra VEGF gene expression on the proliferation of osteoblast cell. METHODS: The extra VEGF gene was induced into osteoblast cell. Then the expression of extra VEGF gene was detected, and its effects on the cell proliferation curve, cell cycle, on the synthesis of type I collagen and on the secretion of osteocalcium of osteoblast cell were investigated. RESULTS: At the first two days of culture, the cell number of pBLAST49-mVEGF gene transfer group was larger than that of the control group, yet no statistically significant difference was seen between the two groups (P > 0.05); however, significant difference was observed from the 3rd day of culture (P < 0.05). The (G2/M+S)% of the 1-5 generation osteoblast cell in pBLAST49-mVEGF gene transfer group was higher than that of the control group, there was significant difference between them (P < 0.05). The concentration of osteocalcium and expression of type I collagen of the 1-5 generation osteoblast cell in pBLAST49-mVEGF gene transfer group were significantly higher than those of the control group (P < 0.05). CONCLUSION: pBLAST49-mVEGF gene transfer can improve the proliferation and function of osteoblast cell.