| 正交设计法优选土茯苓中落新妇苷及总黄酮的提取工艺 |
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| 引用本文： | 林耿丰,梁伟杰,肖凤霞,邓少东,邓超明,黎杏仪. 正交设计法优选土茯苓中落新妇苷及总黄酮的提取工艺[J]. 亚太传统医药, 2011, 7(6): 39-42 |
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| 作者姓名： | 林耿丰 梁伟杰 肖凤霞 邓少东 邓超明 黎杏仪 |
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| 作者单位： | 1. 广东省中医院,广东广州,510120
2. 广州中医药大学中药学院,广东广州,510006 |
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| 摘 要： | 目的:研究超声法提取土茯苓中落新妇苷及总黄酮的最佳工艺条件。方法:采用正交试验法研究落新妇苷和总黄酮的提取工艺,其中落新妇苷含量用HPLC法测定,总黄酮含量用紫外分光光度法测定。结果:土茯苓中落新妇苷最佳提取工艺为加入甲醇30mL,超声提取15min,超声3次。总黄酮的最佳提取工艺为料液比1∶50,加入50%乙醇,超声提取60min。结论:所建立的方法简便、准确、重复性好,可用于土茯苓药材中落新妇苷及总黄酮的含量测定及其质量控制。
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| 关 键 词： | 土茯苓 落新妇苷 总黄酮 正交试验 |
Optimal Extraction of Total Flavones and Astilbin in Smilax Glabra Roxb by Orthogonal Design |
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| Lin gengfeng,Liang weijie,Xiao fengxia,Deng shaodong,Deng chaoming,Li xingyi. Optimal Extraction of Total Flavones and Astilbin in Smilax Glabra Roxb by Orthogonal Design[J]. Asia-Pacific Traditional Medicine, 2011, 7(6): 39-42 |
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| Authors: | Lin gengfeng Liang weijie Xiao fengxia Deng shaodong Deng chaoming Li xingyi |
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| Affiliation: | Lin gengfeng1,Liang weijie2,Xiao fengxia2,Deng shaodong2,Deng chaoming2,Li xingyi2(1.The Guangdong Provincial Hospital of Chinese Traditional Medicine,2.School of Chinese Herbal Medicine,Guangzhou University of TCM,Guangzhou 510006,China) |
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| Abstract: | Objective:To optimize and establish the extraction of total flavones and astilbin in Smilax Glabra Roxb.Methods:The orthogonal design was used in the research on the ultrasonic-assisted technique extraction technique,the content of total flavones and astilbin was determined by UV and HPLC methods.Results:The optimal extraction condition of astilbin was extracting with 30 mL methanol with 15min for 3 times,and the total flavones was extracting with 50 times volume of sample weight with 50% alcohol for 1h.Con... |
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| Keywords: | Smilax Glabra Roxb Astilbin Total Flavones Orthogonal Design |
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