| 紫杉醇、硒酸酯多糖序贯给药对乳腺癌MCF-7细胞抑制作用的研究 |
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| 引用本文： | 陈春影,凌娜,徐艳艳,季宇彬. 紫杉醇、硒酸酯多糖序贯给药对乳腺癌MCF-7细胞抑制作用的研究[J]. 亚太传统医药, 2011, 7(10): 13-15 |
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| 作者姓名： | 陈春影 凌娜 徐艳艳 季宇彬 |
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| 作者单位： | 1. 哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076;国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076
2. 哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨,150076 |
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| 摘 要： | 目的:研究硒酸酯多糖与紫杉醇单独及序贯给药对体外培养乳腺癌MCF-7细胞的生长抑制作用及对细胞周期的影响。方法:采用MTT法及SRB法检测药物对乳腺癌MCF-7细胞体外杀伤作用,应用金氏公式判断联合用药效果,流式细胞仪检测细胞周期及凋亡率。结果:MTT及SRB法结果显示,药物对MCF-7细胞的抑制作用呈剂量依赖性,两者联合作用效果优于单独作用组,30mg/L的硒酸酯多糖与0.5～2nmol/L紫杉醇联合应用表现为协同作用,与4nmol/L紫杉醇联合应用表现为相加作用。流式细胞术结果显示,紫杉醇作用于G1期,同时添加组及序贯给药组既可发生S期和G2/M期周期阻滞,又可诱导凋亡,但凋亡率低于Taxol单独组。结论:紫杉醇与硒酸酯多糖对乳腺癌MCF-7细胞有协同抑制增殖和诱导凋亡的作用。
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| 关 键 词： | 硒酸酯多糖 紫杉醇 乳腺癌MCF-7细胞 凋亡 |
Growth Inhibition of K-selenocarrageenan in Combination with Paclitaxel on Human Breast Carcinoma MCF-7 Cell Line |
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| Chen Chunying,Ling Na,Xu Yanyan,Ji Yubin. Growth Inhibition of K-selenocarrageenan in Combination with Paclitaxel on Human Breast Carcinoma MCF-7 Cell Line[J]. Asia-Pacific Traditional Medicine, 2011, 7(10): 13-15 |
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| Authors: | Chen Chunying Ling Na Xu Yanyan Ji Yubin |
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| Affiliation: | Chen Chunying1,2,Ling Na1,XuYanyan1,Ji Yubin1(1.Life Science and Environment Science Research Center,Harbin University of Commerce,Harbin 150076,China,2.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,China) |
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| Abstract: | Objective:To investigate the inhibitory effect of paclitaxel(taxol) combined with k-selenocarrageenan(KSC) on growth of breast cancer MCF-7 cell. Methods:The inhibitory effects of the drugs on the growth of breast cancer cells were determined by MTT assay and Sulforhodamine B(SRB).The Jin's formula was used to analyze the effect of combined drug therapy.Cell cycle distribution and apoptosis rate were assessed by flow cytometry(FCM). Results:taxol and KSC have obvious growth inhibiting effect on MCF-7 cells ... |
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| Keywords: | K-selenocarrageenan Paclitaxel Breast Cancer MCF-7Cell Apoptosis |
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