脑胶质瘤组织p14^ARF、p53和p21^WAF1的表达及其意义

目的：探讨p14^ARF、p53和p21^WAF1蛋白在不同级别脑胶质瘤组织中的表达及其在胶质瘤发生、发展中的生物学意义。方法：应用免疫组化SP法检测Ⅱ级和Ⅳ级胶质瘤组织中p14^ARF、p53和p21^WAF1蛋白的表达，并分析其与胶质瘤组织学分级之间的关系。结果：Ⅱ级和Ⅳ级胶质瘤中，p53蛋白的阳性表达率分别为28．00％（7／25）及60．87％（14／23）（P=0．022），其阳性表达率随胶质瘤恶性程度的增加而升高，Spearman等级相关分析显示，p53的表达与胶质瘤分级呈正相关（P〈0．05）；p21^WAF1的阳性表达率分别为76．00％（19／25）及39．13％（9／23）（P=0．010），P14^ARF的阳性表达率分别为76．00％（19／25）及34．78％（8／23）（P=0．004），二者阳性表达率均随胶质瘤恶性程度的增加而降低，Spearman等级相关分析显示，p21^WAF1、p14^ARF的表达与胶质瘤分级呈负相关（P〈0．05）。结论 胶质瘤组织中，p53呈不同程度的过表达，且随胶质瘤恶性程度的增加而表达水平升高；p21^WAF1、p14^ARF随胶质瘤恶性程度的增加表达水平降低。突变型p53蛋白的过表达以及p21^WAF1、p14^ARF蛋白的低表达，可促进胶质瘤的发生、发展。

Expression of p14^ARF, p53 and p21^WAF1 protein in gliomas and their significance

Objective：To investigate the expressions of p14^ARF ,p53 and p21^WAF1 proteins in different grades gliomas and their biological significance in tumorigenesis and progression of human gliomas. Methods： The expressions of p14^ARF ,p53 and p21^WAF1 proteins were determined in grade Ⅱ and grade Ⅳ gliomas by immunohistochemical strepta- vidin -peroxidase techniques. And the correlation was analyzed between the expressions of p14^ARF, p53, and p21^WAF1 protein and the grades of gliomas. Results：The positive rates of 1353 proteins were 28.00% （7/25） and 60.87% （14/23） （P = 0. 022 ） in grade Ⅱand grade Ⅳ gliomas, respectively. The positive rates of p53 increased with the elevation of glioma malignancy, and there was a positive correlation between the expressions of p53 and the grades of gliomas by Spearman correlation analysis （P 〈0.05）. The positive rates of p21^WAF1 proteins were 76.00% （19/25） and 39.13% （9/23） （P =0.010） in grade Ⅱ and grade Ⅳ gliomas,respectively. The positive rates of p14^ARF proteins were 76.00% （ 19/25 ） and 34.78% （ 8/23 ） （ P = 0. 004 ） in grade Ⅱ and gradeⅣ gliomas, respectively. The positive rates of p21^WAF1 and p14^ARF decreased with elevated malignant degree in gliomas. There was a negative cone -lation between the expressions of p21^WAF1 and p14^ARF proteins and the grades of gliomas by Spearman correlation analysis （ P 〈 0.05 ）. Conclusion： p53 protein is over - expressed in gliomas to a certain extent and the positive rates of p53 are gradually elevated with the increase of glioma malignancy, but the positive rates of p21^WAF1 and p14^ARF are gradually decreased with elevated histological grades. The over - expression of mutate p53 protein, and the low - expression of p21^WAF1 and p14^ARF might promote the tumorigenesis and progression of gliomas.