| 胆碱能受体激动剂和拮抗剂对体外CFU-GM增殖和分化的影响 |
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| 引用本文： | 邱立波,徐有恒,周衍椒,唐恢玲,蒋德昭.胆碱能受体激动剂和拮抗剂对体外CFU-GM增殖和分化的影响[J].中南大学学报(医学版),1989(3). |
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| 作者姓名： | 邱立波 徐有恒 周衍椒 唐恢玲 蒋德昭 |
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| 作者单位： | 湖南医科大学血液生理研究室 研究生
(邱立波)，湖南医科大学血液生理研究室 导师
(徐有恒，周衍椒，唐恢玲)，湖南医科大学血液生理研究室 导师(蒋德昭) |
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| 摘 要： | 用体外琼脂培养法研究胆碱能受体激动剂和拮抗剂对粒、单造血祖细胞(CFU-GM)增殖和分化的影响。结果显示,10~(-10)-10~(-6)氨甲酰胆碱均能增加CFU-GM的产率,阿托品可以阻断这种效应;氨甲酰胆碱亦能增加CFU*GM的自杀率及培养基中粒细胞集落的比例。文中讨论了CFU-GM上胆碱能受体的功能特征。
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| 关 键 词： | 造血干细胞 氨甲酰胆碱 受体 胆碱能 增殖 细胞分化 小鼠 |
EFFECT OF CHOLINERGIC AGONIST AND ANTAGONIST ON PROLIFERATION AND DIFFERENTIATION OF CFU-GM IN VITRO |
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| QiuLibo XuYouheng Zhou Yanjiao Tang Huiling Jiang DezhaoResearch Laboratory of Blood Physiology,Hunan Medical University.EFFECT OF CHOLINERGIC AGONIST AND ANTAGONIST ON PROLIFERATION AND DIFFERENTIATION OF CFU-GM IN VITRO[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1989(3). |
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| Authors: | QiuLibo XuYouheng Zhou Yanjiao Tang Huiling Jiang DezhaoResearch Laboratory of Blood Physiology Hunan Medical University |
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| Abstract: | This study observed the effect of cholinergic agonist and antagonist on proliferation and differentiation of rganulocytemacrophage haemopoietic progenitor cells (CFU-GM) in vitro. The results showed dosage dependent enhancement of granulocytemacrophage colony growth after treatment of murine bone marrow cultrue with cholinergic agonist carbamylcholine (Cach) .Exposure of CFU-GM to Cach caused a marked increase in their sensitivity to the cytocidal action of cytosine arabinoside.On the other hand, the proportion of granulocytic colonies , which was identified by staining in situ, among the total CFU-GM colonies was also increased after administration Cach. Atropine but not d-Tubocurarine might abolish the above cell effects of Cach.These data indicate the presence of muscarine receptors on CFU-GM. Through activating muscarinic, receptors, Cach may trigger CFU-GM form Go into DNA synthesis phase, and induce differetiation of CFU-GM towards granulocytic cell line. |
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| Keywords: | haematopoietic stem cells carbalchol receptors cholinergic proliferation cell differentiation mice |
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