辛伐他汀对自发性高血压大鼠左心室肥厚作用及心肌转化生长因子-β1表达影响

目的观察辛伐他汀对自发性高血压大鼠左心室肥厚作用及心肌转化生长因子-β1(TGF-β1)表达的的影响。方法实验用WKY大鼠做阴性对照组,SHR大鼠分为对照组和辛伐他汀治疗组。测量大鼠尾动脉收缩压(SBP)及左心室重量指数(LVMI)。应用HE、VG染色、免疫组织化学的方法,结合计算机图像分析技术,检测心肌细胞的直径(TDM)和面积(CA)、心肌组织胶原体积比例(CVF)、血管周围胶原面积和管腔面积比例(PVCA)以及左心室心肌TGF-β1表达。结果辛伐他汀组未能有效降低血压,但可以降低左心室肥厚;SHR辛伐他汀组与SHR对照组相比TDM、CA、CVF、PVCA明显降低,TGF-β1表达下调明显(均P<0.05)。结论应用辛伐他汀治疗可逆转高血压大鼠左心室肥厚形成,TGF-β1可能与辛伐他汀逆转左心室肥厚的机制有关。

Effect of simvastatin on left ventricular hypertrophy and the expression TGF-β1 of myocardium in SHR

Objective To investigate the effects of simvastatin on left ventricular hypertrophy(LVH)and the expression of transforming growth factor-β1(TGF-β1)of myocardium in SHR.Methods Normal wistarkyoto(WKY)rats were used as negative control,and spontaneously hypertensive rats(SHR) were randomly used as positive group and simvastatin group.Systolic blood pressure(SBP)and left ventricular mass index(LVMI)were measured.HE,VG and immunohistochemical staining combined with computed morphometry were imployed to evaluate the cardiomyocyte size,diameter,the collagen volume fraction(CVF),perivascular circumferential area(PVCA),and transforming growth factor-β1(TGF-β1)expression in the left ventricular tissue.Results In simvastatin group,left ventricular mass index(LVMI)were decreased apparently,while systolic blood pressure(SBP)were not affected.Compared to SHR group,cardiomyocyte size,diameter,the collagen volume fraction(CVF) and perivascular circumferential area(PVCA) were significantly reduced as well as the expression of TGF-β1.Conclusion Treatment with simvastatin can inhibit and regress the development of LVH in spontaneously hypertensive rats. TGF-β1 may be involved in the reversal mechanism of LVH by simvastatin.